HLM1, an essential signaling component in the hypersensitive response, is a member of the cyclic nucleotide-gated channel ion channel family

被引:276
作者
Balagué, C
Lin, BQ
Alcon, C
Flottes, G
Malmström, S
Köhler, C
Neuhaus, G
Pelletier, G
Gaymard, F
Roby, D [1 ]
机构
[1] INRA, UMR CNRS, INSERM 215, Lab Biol Mol Relat Plantes Microorganismes, F-31326 Castanet Tolosan, France
[2] Univ Montpellier 2, UMR CNRS, INRA, Lab Biochim & Physiol Mol Plantes, F-34060 Montpellier 1, France
[3] Univ Freiburg, D-79104 Freiburg, Germany
[4] INRA, Lab Genet & Ameliorat Plantes, F-78026 Versailles, France
关键词
D O I
10.1105/tpc.006999
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The hypersensitive response (HR) in plants is a programmed cell death that is commonly associated with disease resistance. A novel mutation in Arabidopsis, hlm1, which causes aberrant regulation of cell death, manifested by a lesion-mimic phenotype and an altered HR, segregated as a single recessive allele. Broad-spectrum defense mechanisms remained functional or were constitutive in the mutant plants, which also exhibited increased resistance to a virulent strain of Pseudomonas syringae pv tomato. In response to avirulent strains of the same pathogen, the hlm1 mutant showed differential abilities to restrict bacterial growth, depending on the avirulence gene expressed by the pathogen. The HLM1 gene encodes a cyclic nucleotide-gated channel, CNGC4. Preliminary study of the HLM1/CNGC4 gene product in Xenopus oocytes (inside-out patch-clamp technique) showed that CNGC4 is permeable to both K+ and Na+ and is activated by both cGMP and cAMP. HLM1 gene expression is induced in response to pathogen infection and some pathogen-related signals. Thus, HLM1 might constitute a common downstream component of the signaling pathways leading to HR/resistance.
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收藏
页码:365 / 379
页数:15
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