Isolation and infusion of donor CD34+ bone marrow cells in cadaver kidney transplantation

被引:12
作者
De Pauw, L
Abramowicz, D
Donckier, V
Kornreich, A
Destree, M
Demoor, F
Andrien, M
Lambermont, M
Goldman, M
机构
[1] Free Univ Brussels, Hop Erasme, Dept Immunol Transfus, B-1070 Brussels, Belgium
[2] Free Univ Brussels, Hop Erasme, Dept Nephrol Dialysis Transplantat, B-1070 Brussels, Belgium
[3] Free Univ Brussels, Hop Erasme, Dept Digest Surg, B-1070 Brussels, Belgium
[4] Cellpro Europe, Wezembeek Oppem, Belgium
关键词
bone marrow; stem cell; kidney transplantation; tolerance;
D O I
10.1093/ndt/13.1.34
中图分类号
R3 [基础医学]; R4 [临床医学];
学科分类号
1001 ; 1002 ; 100602 ;
摘要
Background, Infusion of donor bone marrow cells induces tolerance in allograft models. CD34(+) stem cells present in human bone marrow could be endowed with tolerogenic properties. Methods, CD34(+) stem cells were isolated from bone marrow extracted from vertebral bodies of cadaveric donors. Donor CD34(+) cells (0.6-3.7 x 10(6)/kg) were infused during surgery in 10 kidney transplant recipients receiving OKT3 as induction therapy. Chimerism was investigated using nested PCR for donor-specific HLA alleles. Results. The infusion of CD34(+) stem cells was perfectly tolerated. Five patients remained free of acute rejection at follow-up, 47-325 days post-operatively. The five other patients underwent a single episode of corticosensitive acute rejection. Long-term chimerism was not induced in the seven patients investigated for the persistence of donor DNA. Conclusions, Infusion of donor CD34(+) stem cells in kidney transplantation is safe. The clinical usefulness of the procedure remains to be established.
引用
收藏
页码:34 / 36
页数:3
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