Protectivity of an immunoaffinity-purified 39 kDa capsular protein of avian Pasteurella multocida in mice

被引：19

作者：

Ali, HAH

Sawada, T ^{[1
]}

Noda, K

机构：

[1] Nippon Vet & Anim Sci Univ, Dept Vet Microbiol, Fac Vet Med, Musashino, Tokyo 1188602, Japan

[2] Natl Vet Assay Lab, Kokubunji, Tokyo 1858511, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2004年 / 66卷 / 12期

关键词：

active cross-protection; avian Pasteurella multocida; 39 kDa capsular protein;

D O I：

10.1292/jvms.66.1603

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 [兽医学];

摘要：

A 39 kDa protein of avian Pasteurella multocida strain P-1059 (serovar A:3) was purified from a crude capsular extract by inummoaffinity chromatography by using a ligand of purified mouse monoclonal antibody to the 39 kDa capsular protein of the strain. Protective activity of the purified 39 kDa protein antigen was determined by inoculation of ddY mice twice with 25 or 125 mug of the protein and challenge-exposure with 10 or 50 LD50 of strains P-1059 or X-73 (serovar A: 1). The results showed that the antigen gave high protection (60 to 100%). These results indicated that the 39 kDa protein of avian P. multocida is a cross-protective antigen over serovars A:1 and A:3.

引用

页码：1603 / 1604

页数：2

共 13 条

[1]

Characterization of a 39 kDa capsular protein of avian Pasteurella multocida using monoclonal antibodies [J].