Derivatization for electrospray ionization mass spectrometry. 3. Electrochemically ionizable derivatives

被引:156
作者
Van Berkel, GJ [1 ]
Quirke, JME
Tigani, RA
Dilley, AS
Covey, TR
机构
[1] Oak Ridge Natl Lab, Div Chem & Analyt Sci, Oak Ridge, TN 37831 USA
[2] Florida Int Univ, Dept Chem, Miami, FL 33199 USA
[3] SCIEX MDS Hlth Grp, Concord, ON L4K 4V8, Canada
关键词
D O I
10.1021/ac971348o
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
In this paper, the use of ferrocene-based "electrochemically ionizable" derivatives to enhance ES-MS analysis of simple alcohols, sterols, and phenols is discussed. These derivatives are designed to take advantage of the electrolysis process inherent to operation of the ES ion source for selective ionization. Derivatization procedures, electrochemical character of the derivatives, and the ES-MS operational parameters necessary to maximize electrochemical ionization and to enhance gas-phase detection are presented with reference to ferrocenecarbamate ester derivatives of a variety of alcohol standards, as well as the ferroceneboronate derivative of the diol, pinacol. Tandem mass spectrometric analysis of the derivatives (precursor and product ion spectra) is shown to provide derivative confirmation, enhanced detection, and additional analyte structure information. The utility of this derivatization approach for the selective detection of alcohols in complicated mixtures is demonstrated using a saw palmetto (Serenoa repens) fruit extract known to contain a variety of alcohols at low levels.
引用
收藏
页码:1544 / 1554
页数:11
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