Unnatural ligands for engineered proteins: New tools for chemical genetics

被引：139

作者：

Bishop, A ^{[1
]}

Buzko, O ^{[1
]}

Heyeck-Dumas, S ^{[1
]}

Jung, I ^{[1
]}

Kraybill, B ^{[1
]}

Liu, Y ^{[1
]}

Shah, K ^{[1
]}

Ulrich, S ^{[1
]}

Witucki, L ^{[1
]}

Yang, F ^{[1
]}

Zhang, C ^{[1
]}

Shokat, KM ^{[1
]}

机构：

[1] Princeton Univ, Dept Chem, Princeton, NJ 08544 USA

来源：

ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE | 2000年 / 29卷

关键词：

protein engineering; orthogonal ligands; signal transduction; protein design;

D O I：

10.1146/annurev.biophys.29.1.577

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Small molecules that modulate the activity of biological signaling molecules can be powerful probes of signal transduction pathways. Highly specific molecules with high affinity are difficult to identify because of the conserved nature of many protein active sites. A newly developed approach to discovery of such small molecules that relies on protein engineering and chemical synthesis has yielded powerful tools for the study of a wide variety of proteins involved in signal transduction (G-proteins, protein kinases, 7-transmembrane receptors, nuclear hormone receptors, and others). Such chemical genetic tools combine the advantages of traditional genetics and the unparalleled temporal control over protein function afforded by small molecule inhibitors/activators that act at diffusion controlled rates with targets.

引用

页码：577 / 606

页数：40

共 72 条

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