Structure and stress-related expression of two cDNAs encoding proteinase inhibitor II of Nicotiana glutinosa L.

被引：32

作者：

Choi, D

Park, JA

Seo, YS

Chun, YJ

Kim, WT ^{[1
]}

机构：

[1] Yonsei Univ, Coll Sci, Dept Biol, Seoul 120749, South Korea

[2] Korea Res Inst Biosci & Biotechnol, Plant Cell Biotechnol Lab, Taejeon 305600, South Korea

[3] Yonsei Univ, Coll Sci, Inst Life Sci, Seoul 120749, South Korea

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2000年 / 1492卷 / 01期

关键词：

cDNA; proteinase inhibitor; stress-induced gene expression; tissue-specific expression; tobacco mosaic virus; Nicotiana glutinosa L;

D O I：

10.1016/S0167-4781(00)00073-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two cDNAs, pNGPI-1 and pNGPI-2, encoding Nicotiana glutinosa proteinase inhibitor II (PI-II) have been cloned, sequenced and identified. The deduced amino acid sequences are 54-82% identical to those of other plant PI-II. The NGPI-1 protein is composed of eight repeated domains, while NGPI-2 contains six repeated regions, each with a putative reactive site. The expression of NGPI-1 is highly regulated in a developmental- and tissue-specific manner, with the transcript being detected in young leaves and floral organs of N. glutinosa plants. In mature leaves, the NGPI-1 gene is rapidly activated by distinct temporal induction patterns in response to pathogen-related (biotic) and wound-related (abiotic) stresses. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：211 / 215

页数：5

共 18 条

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