Differential expression of multidrug resistance genes in naive rat brain

被引：33

作者：

Kwan, P

Sills, GJ ^{[1
]}

Butler, E

Gant, TW

Brodie, MJ

机构：

[1] Univ Glasgow, Western Infirm, Dept Med & Therapeut, Epilepsy Unit, Glasgow G11 6NT, Lanark, Scotland

[2] Univ Leicester, MRC, Toxicol Unit, Leicester, Leics, England

来源：

NEUROSCIENCE LETTERS | 2003年 / 339卷 / 01期

关键词：

multidrug resistance; P-glycoprotein; quantitative reverse-transcriptase polymerase chain reaction; brain; rat;

D O I：

10.1016/S0304-3940(02)01453-2

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The multidrug resistance (mdr1) gene family encodes the efflux transporter P-glycoprotein (P-gp) which contributes to the functionality of the blood-brain barrier. We have characterised the regional expression of mdr1 genes in naive rat brain. Adult male Sprague-Dawley rats (n = 6) were sacrificed and their brains rapidly removed. Seven distinct anatomical regions were isolated by microdissection and the expression of mdr1a and mdr1b determined by quantitative reverse-transcriptase polymerase chain reaction. The mdr1a isoform was expressed in all brain regions investigated, while mdr1b was expressed to a quantifiable degree in hippocampus alone. These findings reveal a differential expression of mdr1 genes in normal rodent brain tissue and suggest that P-gp may afford a broader protection of the hippocampus than other brain structures. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：33 / 36

页数：4

共 20 条

[1] Overexpression of multiple drug resistance genes in endothelial cells from patients with refractory epilepsy [J].