BMP-4 Response in Wild-Type and Craniosynostotic Rabbit Bone Cells

被引:8
作者
Cooper, Gregory M.
Lensie, Emily L.
Cray, James J., Jr.
DeCesare, Gary E.
Smalley, Melissa A.
Losee, Joseph E.
Mooney, Mark P.
机构
[1] Univ Pittsburgh, Dept Surg, Div Plast & Reconstruct Surg, Pittsburgh, PA USA
[2] Univ Pittsburgh, Dept Oral Biol, Pittsburgh, PA USA
[3] Univ Pittsburgh, Dept Bioengn, Pittsburgh, PA USA
[4] Univ Pittsburgh, Dept Anthropol, Pittsburgh, PA USA
[5] Univ Pittsburgh, Dept Orthodont, Pittsburgh, PA USA
[6] Pediat Craniofacial Biol Lab, Pittsburgh, PA USA
基金
美国国家卫生研究院;
关键词
CORONAL SUTURE SYNOSTOSIS; INHIBITS POSTOPERATIVE RESYNOSTOSIS; IN-VITRO DIFFERENTIATION; GROWTH-FACTOR RECEPTOR-2; DURA-MATER; OSSEOUS OBLITERATION; TISSUE INTERACTIONS; EXPRESSION; MATRIX; PATHOGENESIS;
D O I
10.1097/PRS.0b013e3181d62ad4
中图分类号
R61 [外科手术学];
学科分类号
100210 [外科学];
摘要
Background: Craniosynostosis results from improper regulation of bone formation. Investigations of cells derived from patients with craniosynostosis suggest that craniosynostotic bone-derived cells have increased osteogenic or proliferative capacities compared with other cells. Research into the pathogenesis of craniosynostosis using cells derived from children has been hindered by small sample sizes and inappropriate control cell populations. The authors hypothesized that cells derived from suture-associated regions of bone from craniosynostotic rabbits were more osteogenic and proliferative than bone cells derived from wild-type rabbits. Methods: This study used cells derived from a colony of rabbits with congenital, nonsyndromic craniosynostosis (n = 20) or from age-matched wild-type rabbits (n = 20). Bone cells derived from either suture-associated or non-suture-associated bone were challenged with osteogenic stimuli and assessed for osteogenic differentiation. Results: The results suggest a high level of variability among cells derived from different individual rabbits. Also, craniosynostotic bone cells have a larger response to recombinant human bone morphogenetic protein 4 stimulation relative to baseline expression of alkaline phosphatase, although overall alkaline phosphatase expression was higher in wild-type bone cells. Cell proliferation showed some differences at 3 clays in culture, but no differences were found at 7 clays in culture. Conclusions: This study suggests that bone cells in this rabbit model of craniosynostosis are generally similar to wild-type cells. Also, because of variability, it is necessary to have larger sample sizes than are normally available in human studies. Therefore, cells from the rabbit model may be a powerful in vitro model for further craniosynostosis research. (Plast. Reconstr. Slog. 125: 1403, 2010.)
引用
收藏
页码:1403 / 1411
页数:9
相关论文
共 34 条
[1]
P253R fibroblast growth factor receptor-2 mutation induces RUNX2 transcript variants and calvarial osteoblast differentiation [J].
Baroni, T ;
Carinci, P ;
Lilli, C ;
Bellucci, C ;
Aisa, MC ;
Scapoli, L ;
Volinia, S ;
Carinci, F ;
Pezzetti, F ;
Calvitti, M ;
Farina, A ;
Conte, C ;
Bodo, M .
JOURNAL OF CELLULAR PHYSIOLOGY, 2005, 202 (02) :524-535
[2]
Apert's syndrome: Differential in vitro production of matrix macromolecules and its regulation by interleukins [J].
Bodo, M ;
Carinci, F ;
Baroni, T ;
Giammarioli, M ;
Bellucci, C ;
Bosi, G ;
Pezzetti, F ;
Becchetti, E ;
Evangelisti, R ;
Carinci, P .
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, 1997, 27 (01) :36-42
[3]
CROUZONS-DISEASE CORRELATES WITH LOW FIBROBLASTIC GROWTH-FACTOR RECEPTOR ACTIVITY IN STENOSED CRANIAL SUTURES [J].
BRESNICK, S ;
SCHENDEL, S .
JOURNAL OF CRANIOFACIAL SURGERY, 1995, 6 (03) :245-248
[4]
Expression profiles of craniosynostosis-derived fibroblasts [J].
Carinci, F ;
Bodo, M ;
Tosi, L ;
Francioso, F ;
Evangelisti, R ;
Pezzetti, F ;
Scapoli, L ;
Martinelli, M ;
Baroni, T ;
Stabellini, G ;
Carinci, P ;
Bellucci, C ;
Lilli, C ;
Volinia, S .
MOLECULAR MEDICINE, 2002, 8 (10) :638-644
[5]
Carinci P, 2000, INT J DEV BIOL, V44, P715
[6]
Noggin inhibits postoperative resynostosis in craniosynostotic rabbits [J].
Cooper, Gregory M. ;
Curry, Chris ;
Barbano, Timothy E. ;
Burrows, Anne M. ;
Vecchione, Lisa ;
Caccamese, John F. ;
Norbutt, Craig S. ;
Costello, Bernard J. ;
Losee, Joseph E. ;
Moursi, Amr M. ;
Huard, Johnny ;
Mooney, Mark P. .
JOURNAL OF BONE AND MINERAL RESEARCH, 2007, 22 (07) :1046-1054
[7]
Identification of genes differentially expressed by prematurely fused human sutures using a novel in vivo-in vitro approach [J].
Coussens, Anna K. ;
Hughes, Ian P. ;
Wilkinson, Christopher R. ;
Morris, C. Phillip ;
Anderson, Peter J. ;
Powell, Barry C. ;
van Daal, Angela .
DIFFERENTIATION, 2008, 76 (05) :531-545
[8]
Unravelling the molecular control of calvarial suture fusion in children with craniosynostosis [J].
Coussens, Anna K. ;
Wilkinson, Christopher R. ;
Hughes, Ian P. ;
Morris, C. Phillip ;
van Daal, Angela ;
Anderson, Peter J. ;
Powell, Barry C. .
BMC GENOMICS, 2007, 8 (1)
[9]
In Vitro Differentiation of Human Calvarial Suture Derived Cells With and Without Dexamethasione Does Not Induce In Vivo-Like Expression [J].
Coussens, Anna K. ;
Hughes, Ian P. ;
Morris, C. Phillip ;
Powell, Barry C. ;
Anderson, Peter J. .
JOURNAL OF CELLULAR PHYSIOLOGY, 2009, 218 (01) :183-191
[10]
DePollack C, 1996, J BONE MINER RES, V11, P401