Virus a la sauce Yo-Pro: Microwave-enhanced staining for counting viruses by epifluorescence microscopy

被引：36

作者：

Xenopoulos, MA ^{[1
]}

Bird, DF ^{[1
]}

机构：

[1] Univ Quebec, Dept Sci Biol, Montreal, PQ H3C 3P8, Canada

来源：

LIMNOLOGY AND OCEANOGRAPHY | 1997年 / 42卷 / 07期

关键词：

D O I：

10.4319/lo.1997.42.7.1648

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A recently developed method for enumerating aquatic viruses (Yo-Pro stained) was modified to reduce staining time and allow counting of fixed samples. Marine and freshwater virus samples to be enumerated were stained with the cyanine-based dye Yo-Pro-1 by using microwave irradiation, which reduced incubation time from 48 h to 4 min. The modified method and the original protocol yielded similar viral estimates. Easier sample handling and the addition of fixed-sample capability should allow epifluorescence counting of viruses to become a routine part of field studies of viral ecology.

引用

页码：1648 / 1650

页数：3

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