Immunomodulating effects of cefodizime on Klebsiella pneumoniae-stimulated neutrophils

被引：13

作者：

Song, H ^{[1
]}

Li, GW ^{[1
]}

Ye, J ^{[1
]}

Wan, F ^{[1
]}

Qian, YS ^{[1
]}

机构：

[1] Shantou Univ, Coll Med, Dept Pharmacol & Tumor Hosp, Guangdong 515041, Peoples R China

来源：

IMMUNOBIOLOGY | 2004年 / 209卷 / 03期

关键词：

neutrophil; cefodizime; TNF-alpha; IL-1; beta; NF-kappa B;

D O I：

10.1016/j.imbio.2004.05.003

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

To explore the immunoregulating effects of cefodizime on neutrophils and its mechanisms, we detected the expression of some cytokines secreted by neutrophils after heat-killed Klebsiella pneumoniae induced inflammation. We analyzed the changes of signal transduction in this process by detecting the mRNA expression of toll like receptor 4 (TLR4) and the inhibitor factor of kappaBalpha (I-kappaBalpha) expressed by neutrophils. The activity of nuclear factor kappaB (NF-kappaB) in neutrophils was also assayed by electrophoretic mobility shift assay (EMSA). We found cefodizime increased neutrophil production of TNF-alpha, IL-1beta in the early stage of inflammation, which was in agreement with the enhanced mRNA expression of TLR4 and DNA-binding activity of NF-kappaB. Taken together, the immunomodulating effects of cefodizime on cytokine production of K pneumoniae stimulated neutrophils is possibly due to its regulation of TLR4 mRNA expression and DNA binding activities of NF-kappaB through the LPS-TLR4-NF-kappaB pathway. (C) 2004 Elsevier GmbH. All rights reserved.

引用

页码：277 / 282

页数：6

共 23 条

[1] Immunopharmacology of rapamycin [J].

Abraham, RT ;

Wiederrecht, GJ .

ANNUAL REVIEW OF IMMUNOLOGY, 1996, 14 :483-510

[2]

ARAI A, 1996, MICROBIOL IMMUNOL, V40, P333

[3] IκB-NF-κB structures:: At the interface of inflammation control [J].

Baeuerle, PA .

CELL, 1998, 95 (06) :729-731

[4] Mechanisms of disease - Nuclear factor-kappa b - A pivotal transcription factor in chronic inflammatory diseases [J].

Barnes, PJ ;

Larin, M .

NEW ENGLAND JOURNAL OF MEDICINE, 1997, 336 (15) :1066-1071

[5] Reduction by cefodizime of the pulmonary inflammatory response induced by heat-killed Streptococcus pneumoniae in mice [J].

Bergeron, Y ;

Ouellet, N ;

Deslauriers, AM ;

Simard, M ;

Olivier, M ;

Bergeron, MG .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1998, 42 (10) :2527-2533

[6] Influence of cefodizime on pulmonary inflammatory response to heat-killed Klebsiella pneumoniae in mice [J].

Bergeron, Y ;

Deslauriers, AM ;

Ouellet, N ;

Gauthier, MC ;

Bergeron, MG .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (09) :2291-2294

[7] Neutrophils play a critical role in the pathogenesis of experimental cerebral malaria [J].

Chen, L ;

Zhang, ZH ;

Sendo, F .

CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 2000, 120 (01) :125-133

[8] Cytokine and chemokine mRNA expression in neutrophils from CBA/NSlc mice infected with Plasmodium berghei ANKA that induces experimental cerebral malaria [J].

Chen, L ;

Sendo, F .

PARASITOLOGY INTERNATIONAL, 2001, 50 (02) :139-143

[9]

Chen L, 2001, EUR J IMMUNOL, V31, P265, DOI 10.1002/1521-4141(200101)31:1<265::AID-IMMU265>3.3.CO

[10]

2-C

← 1 2 3 →