Substrate specificity of cellobiose dehydrogenase from Phanerochaete chrysosporium

被引：77

作者：

Henriksson, G ^{[1
]}

Sild, V ^{[1
]}

Szabó, IJ ^{[1
]}

Pettersson, G ^{[1
]}

Johansson, G ^{[1
]}

机构：

[1] Univ Uppsala, Ctr Biomed, Dept Biochem, S-75123 Uppsala, Sweden

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1998年 / 1383卷 / 01期

关键词：

cellobiose dehydrogenase; kinetics; substrate specificity; subsite; cellobiose; glucose; (P-chrysosporium);

D O I：

10.1016/S0167-4838(97)00180-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Substrate structural mapping suggests that the catalytic site of cellobiose dehydrogenase from Phanerochaete chrysosporium forms a narrow cave with two hexose binding subsites, Kinetic data also show that beta-di or oligosaccharides are favored electron donors with respect to both K-M and k(cat). Surprisingly, thiocellobiose showed an even higher k(cat) than cellobiose, although the K-M value was somewhat higher. The CDH was purified using an updated protocol. (C) 1998 Elsevier Science B.V.

引用

页码：48 / 54

页数：7

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