Determination of estrogens and their conjugates in water using solid-phase extraction followed by liquid chromatography-tandem mass spectrometry

被引：188

作者：

Isobe, T

Shiraishi, H

Yasuda, M

Shinoda, A

Suzuki, H

Morita, M

机构：

[1] Natl Inst Environm Studies, Endocrine Disrupters Res Lab, Tsukuba, Ibaraki 3058506, Japan

[2] Showa Denko Co Ltd, Kawasaki Ku, Kawasaki, Kanagawa 2100867, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 2003年 / 984卷 / 02期

关键词：

water analysis; steroids; estrogens; endocrine disrupters;

D O I：

10.1016/S0021-9673(02)01851-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An analytical procedure for the determination of steroid estrogens and their conjugates was developed and applied to aqueous environmental samples. The analytes of 15 compounds were solid-phase extracted and fractionated into two fractions: one containing unconjugated (free) steroids and the other containing conjugates (sulfates and glucuronides). Identification and quantification were Carried out using liquid chromatography coupled with tandem mass spectrometry. The recoveries for each compound ranged from 57 to 116% and reproducibilities represented as RSD ranged from 2.9 to 17%. Some of the sulfates and free compounds were detected in environmental samples, whereas most of the conjugates were below the detection limits. (C) 2002 Elsevier Science B.V. All rights reserved.

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页码：195 / 202

页数：8

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