Sequence of the gene encoding a plasmid-mediated cefotaxime-hydrolyzing class A β-lactamase (CTX-M-4):: Involvement of serine 237 in cephalosporin hydrolysis

被引：82

作者：

Gazouli, M

Tzelepi, E

Sidorenko, SV

Tzouvelekis, LS

机构：

[1] Hellenic Pasteur Inst, Dept Bacteriol, Athens 11521, Greece

[2] Natl Res Ctr Antibiot, Moscow, Russia

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1998年 / 42卷 / 05期

关键词：

D O I：

10.1128/AAC.42.5.1259

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The sequence of the gene encoding a novel cefotaxime-hydrolyzing beta-lactamase (CTX-M-4) was determined. It was located in a plasmid harbored by a Salmonella typhimurium strain. CTX-M-4 was similar to the plasmidic cefotaxime-hydrolyzing beta-lactamases CTX-M-2 and Toho-1 and related to the chromosomal beta-lactamase of Klebsiella oxytoca. A Ser-237-->Ala substitution, introduced by site-directed mutagenesis, caused minor alterations in the interaction of CTX-M-4 with beta-lactams, reducing slightly the relative hydrolytic activity against cefotaxime and the susceptibility to inhibition by clavulanate.

引用

页码：1259 / 1262

页数：4

共 19 条

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