Effect of colloidal gold size on the conformational changes of adsorbed cytochrome c:: Probing by circular dichroism, UV-visible, and infrared spectroscopy

被引：152

作者：

Jiang, X ^{[1
]}

Jiang, UG ^{[1
]}

Jin, YD ^{[1
]}

Wang, EK ^{[1
]}

Dong, SJ ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China

来源：

BIOMACROMOLECULES | 2005年 / 6卷 / 01期

关键词：

D O I：

10.1021/bm049744l

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The conformational changes of bovine heart cytochrome c (cyt c) induced by the adsorption on gold nanoparticles with different sizes have been investigated by electronic absorption, circular dichroism (CD), and Fourier transform infrared spectra. The combination of these techniques can give complementary information about adsorption-induced conformational changes. The results show that there are different conformational changes for cyt c adsorbed on gold nanoparticles with different sizes due to the different interaction forces between cyt c and gold nanoparticles. The colloidal gold concentration-dependent conformation distribution curves of cyt c obtained by analysis of CD spectra using the singular value decomposition least-squares method show that the coverage of cyt c on the gold nanoparticles surface also affects the conformational changes of the adsorbed cyt c.

引用

页码：46 / 53

页数：8

共 52 条

[1] Perspectives on the physical chemistry of semiconductor nanocrystals [J].

Alivisatos, AP .

JOURNAL OF PHYSICAL CHEMISTRY, 1996, 100 (31) :13226-13239

[2] Nanocrystalites: Building blocks for quantum dot heterostructures [J].

Bawendi, MG .

SOLID STATE COMMUNICATIONS, 1998, 107 (11) :709-709

[3] Adsorption to silica nanoparticles of human carbonic anhydrase II and truncated forms induce a molten-globule-like structure [J].

Billsten, P ;

Freskgard, PO ;

Carlsson, U ;

Jonsson, BH ;

Elwing, H .

FEBS LETTERS, 1997, 402 (01) :67-72

[4] Self-assembly of nanoparticles into structured spherical and network aggregates [J].

Boal, AK ;

Ilhan, F ;

DeRouchey, JE ;

Thurn-Albrecht, T ;

Russell, TP ;

Rotello, VM .

NATURE, 2000, 404 (6779) :746-748

[5] Morphology-dependent electrochemistry of cytochrome c at Au colloid-modified SnO2 electrodes [J].

Brown, KR ;

Fox, AP ;

Natan, MJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (05) :1154-1157

[6] Folding of apocytochrome c in lipid micelles:: Formation of α-helix precedes membrane insertion [J].

Bryson, EA ;

Rankin, SE ;

Carey, M ;

Watts, A ;

Pinheiro, TJT .

BIOCHEMISTRY, 1999, 38 (30) :9758-9767

[7] Quantum dot bioconjugates for ultrasensitive nonisotopic detection [J].

Chan, WCW ;

Nie, SM .

SCIENCE, 1998, 281 (5385) :2016-2018

[8]

Chen YL, 1999, J COLLOID INTERF SCI, V214, P38

[9] Intermolecular β-sheet results from trifluoroethanol-induced nonnative α-helical structure in β-sheet predominant proteins:: Infrared and circular dichroism spectroscopic study [J].

Dong, A ;

Matsuura, J ;

Manning, MC ;

Carpenter, JF .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1998, 355 (02) :275-281

[10] REDOX-DEPENDENT CHANGES IN BETA-EXTENDED CHAIN AND TURN STRUCTURES OF CYTOCHROME-C IN WATER SOLUTION DETERMINED BY 2ND DERIVATIVE AMIDE-I INFRARED-SPECTRA [J].

DONG, A ;

HUANG, P ;

CAUGHEY, WS .

BIOCHEMISTRY, 1992, 31 (01) :182-189

← 1 2 3 4 5 6 →