Cultivation of endothelial cells on adhesive protein-free synthetic polymer gels

被引：79

作者：

Chen, YM

Shiraishi, N

Satokawa, H

Kakugo, A

Narita, T

Gong, JP

Osada, Y

Yamamoto, K

Ando, J

机构：

[1] Hokkaido Univ, Grad Sch Sci, Kita Ku, Sapporo, Hokkaido 0600810, Japan

[2] Univ Tokyo, Grad Sch Med, Tokyo 1130033, Japan

来源：

BIOMATERIALS | 2005年 / 26卷 / 22期

关键词：

hydrogel; biocompatibility; cell proliferation; cell culture; tissue engineering; polymer;

D O I：

10.1016/j.biomaterials.2004.11.025

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Various hydrogels without modification by any cell adhesive proteins have been investigated as cell scaffolds. The present study shows that bovine fetal aorta endothelial cells can adhere, spread, proliferate, and reach confluence on poly(acrylic acid), poly(sodium p-styrene sulfonate), and poly(2-acrylamido-2- methyl-l-propanesulfonic sodium) gels, whereas cells reach subconfluence on poly(vinyl alcohol) and poly(meth acrylic acid) gels. The proliferation behavior was sensitive to both hydrogel charge density and crosslinker concentration. The relationship between cell proliferation and zeta potential of gels was discussed. It was found that hydrogels with a negative zeta potential higher than about 20mV facilitates cell proliferation. (c) 2004 Elsevier Ltd. All rights reserved.

引用

页码：4588 / 4596

页数：9

共 29 条

[1] THE EFFECT OF FLUID SHEAR-STRESS ON THE MIGRATION AND PROLIFERATION OF CULTURED ENDOTHELIAL-CELLS [J].