Methylation of CpG dinucleotides and/or CCWGG motifs at the promoter of TP53 correlates with decreased gene expression in a subset of acute lymphoblastic leukemia patients

被引：63

作者：

Agirre, X ^{[1
]}

Vizmanos, JL ^{[1
]}

Calasanz, MJ ^{[1
]}

García-Delgado, M ^{[1
]}

Larráyoz, MJ ^{[1
]}

Novo, FJ ^{[1
]}

机构：

[1] Univ Navarra, Sch Sci, Dept Genet, E-31080 Pamplona, Navarra, Spain

来源：

ONCOGENE | 2003年 / 22卷 / 07期

关键词：

TP53; methylation analysis; promoter analysis; gene expression; ALL;

D O I：

10.1038/sj.onc.1206236

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It has been shown that methylation of CpG dinucleotides located in the promoter region of TP53 is associated with low expression levels of this gene. We have analysed the methylation status of one CpG dinucleotide and of three CCWGG motifs, also located in the promoter region of the gene, in bone marrow samples obtained from patients with acute lymphoblastic leukemia (ALL). Eight out of 25 samples analysed showed methylation of either the CpG dinucleotide, the CCWGG motifs or both. Relative to nonmethylated leukemia samples, TP53 expression levels were decreased in all methylated samples in which TP53 expression could be measured. Methylation of CpG and CCWGG motifs in the promoter of TP53 could represent a novel mechanism leading to functional impairment of this tumor suppressor gene in ALL.

引用

页码：1070 / 1072

页数：3

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