A rapid, quantitative assay for direct detection of microRNAs and other small RNAs using splinted ligation

被引：58

作者：

Maroney, Patricia A.

Chamnongpol, Sangpen

Souret, Frederic

Nilsen, Timothy W.

机构：

[1] Case Western Reserve Univ, Sch Med, Ctr RNA Mol Biol, Cleveland, OH 44106 USA

[2] Case Western Reserve Univ, Sch Med, Dept Biochem, Cleveland, OH 44106 USA

[3] USB Corp, Cleveland, OH 44128 USA

来源：

RNA | 2007年 / 13卷 / 06期

关键词：

miRNA; microRNA; small RNA; splinted ligation; Northern blot;

D O I：

10.1261/rna.518107

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The discovery and characterization of microRNAs (miRNAs) and other families of short RNAs has led to a rapid expansion of research directed at elucidating their expression patterns and regulatory functions. Here, we describe a convenient, sensitive, and straightforward method to detect and quantitate specific miRNA levels in unfractionated total RNA samples. The method, based on splinted ligation, does not require specialized equipment or any amplification step, and is significantly faster and more sensitive than Northern blotting. We demonstrate that the method can be used to detect various classes of small regulatory RNAs from different organisms.

引用

收藏

页码：930 / 936

页数：7

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