A novel octameric AMP-forming acetyl-CoA synthetase from the hyperthermophilic crenarchaeon Pyrobaculum aerophilum

被引：26

作者：

Bräsen, C

Urbanke, C

Schönheit, P

机构：

[1] Univ Kiel, Inst Allgemeine Mikrobiol, D-24118 Kiel, Germany

[2] Med Hochschule Hannover, Inst Biophys Biochem Verfahren, D-30625 Hannover, Germany

来源：

FEBS LETTERS | 2005年 / 579卷 / 02期

关键词：

AMP-forming acetyl-CoA synthetase; hyperthermophilic archaea; Pyrobaculum aerophilum; thermoadaptation;

D O I：

10.1016/j.febslet.2004.12.016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

AMP-forming acetyl-CoA synthetases (ACSs) are ubiquitous in all three domains of life. Here, we report the first characterization of an ACS from a hyperthermophilic organism, from the archaeon Pyrobaculum aerophilum. The recombinant ACS, the gene product of ORF PAE2867, showed extremely high thermostability and thermoactivity at temperatures around 100 degreesC. In contrast to known monomeric or homodimeric mesophilic ACSs, the P. aerophilum ACS was a 610 kDa homooctameric protein, with a significant lower content of thermolabile (Cys, Asn, and Gin) and higher content of charged (Gin, Lys, and Arg) amino acids. Kinetic analyses revealed an unusual broad substrate spectrum for organic acids and an extremely high affinity for acetate (Km 3 muM). (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：477 / 482

页数：6

共 26 条

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