Dipeptidyl peptidase IV in C6 rat glioma cell line differentiation

被引:20
作者
Sedo, A [1 ]
Malík, R
Krepela, E
机构
[1] Charles Univ, Fac Med 1, Inst Med Chem & Biochem 1, Prague 12108, Czech Republic
[2] Acad Sci Czech Republ, Inst Expt Med, Lab Cell Pathol, Prague 12800, Czech Republic
[3] Med Fac Hosp Bulovka, Clin Pneumol & Chest Surg, Dept Mol & Cellular Pneumol, Prague 18081, Czech Republic
关键词
cell differentiation; cell proliferation; glioma; protease;
D O I
10.1515/bchm.1998.379.1.39
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The C6 rat glioma cell line is broadly used as a model in studies of glial cell differentiation. In the present study we demonstrated a significantly higher total cellular, but especially membrane-associated, activity of dipeptidyl peptidase IV in differentiated C6 cells in comparison with their proliferating counterparts. The majority, but not all, of enzyme isoelectric focusing isoforms from differentiated C6 cells displayed a substantially higher activity compared to the proliferating cells, with G-P-NHMec as the substrate. Non-denaturing polyacrylamide gradient gel electrophoresis showed the presence of one major peak of activity, dipeptidyl peptidase IV (M-r of about 220000), in both proliferating and differentiated C6 cells. The results indicate that dipeptidyl peptidase IV regulation is associated with C6 rat glioma cell differentiation.
引用
收藏
页码:39 / 44
页数:6
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