Total serum protein N-glycome profiling on a capillary electrophoresis-microfluidics platform

被引：47

作者：

Callewaert, N

Contreras, R

Mitnik-Gankin, L

Carey, L

Matsudaira, P

Ehrlich, D

机构：

[1] Whitehead Inst Biomed Res, BioMEMS Lab, Cambridge, MA 02142 USA

[2] State Univ Ghent, Dept Mol Biomed Res, Ghent, Belgium

[3] VIB, Ghent, Belgium

来源：

ELECTROPHORESIS | 2004年 / 25卷 / 18-19期

关键词：

biomarkers; cross-injector; glycomics; lab-on-a-chip; microfluidics; miniaturization;

D O I：

10.1002/elps.200406020

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We implemented 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled asparagine-linked glycan (N-glycan) profiling on a microfluidic electrophoresis platform. Using 11.5 cm effective length etched channels and 4% linear polyacrylamide as the separation matrix, the major N-glycans in human serum were profiled in 12 min with a resolution comparable to what is achieved for these analytes on gel-based DNA sequencers. This demonstration suggests a practical clinical application for highspeed compact analyzers which might be uniquely based on microfluidic devices.

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收藏

页码：3128 / 3131

页数：4

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