Proteomic analysis of Schistosoma mansoni egg secretions

被引:174
作者
Cass, Cynthia L.
Johnson, Jeffrey R.
Califf, Lindsay L.
Xu, Tao
Hernandez, Hector J.
Stadecker, Miguel J.
Yates, John R., III
Williams, David L. [1 ]
机构
[1] Illinois State Univ, Dept Biol Sci, Normal, IL 61790 USA
[2] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
[3] Tufts Univ, Sch Med, Dept Pathol, Boston, MA 02111 USA
关键词
Schistosoma mansoni; egg secretome; egg secreted proteins; MudPIT proteomics;
D O I
10.1016/j.molbiopara.2007.06.002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Schistosomiasis remains a largely neglected, global health problem. The morbid pathology of the disease stems from the host's inflammatory response to parasite eggs trapped in host tissues. Long term host/parasite survival is dependent upon the successful modulation of the acute pathological response, which is induced by egg antigens. In this study, using Multidimensional Protein Identification Technology, we identified the Schistosoma mansoni egg secretome consisting of 188 proteins. Notably we identified proteins involved in redox balance, molecular chaperoning and protein folding, development and signaling, scavenging and metabolic pathways, immune response modulation, and 32 novel, previously uncharacterized schistosome proteins. We localized a subset of previously characterized schistosome proteins identified in egg secretions in this study, to the surface of live S. mansoni eggs using the circumoval precipitin reaction. The identification of proteins actively secreted by live schistosome eggs provides important new information for understanding immune modulation and the pathology of schistosomiasis. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:84 / 93
页数:10
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