Differentiation of Chlamydia species by combined use of polymerase chain reaction and restriction endonuclease analysis

被引:18
作者
Yoshida, H
Kishi, Y
Shiga, S
Hagiwara, T
机构
[1] Fuso Pharmaceut Ind Ltd, Ctr Res & Dev, Joto Ku, Osaka 5368523, Japan
[2] Natl Inst Infect Dis, Dept Virol 1, Tokyo 1628640, Japan
关键词
Chlamydia species; differentiation; PCR;
D O I
10.1111/j.1348-0421.1998.tb02303.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
To differentiate Chlamydia spp., a primer pair designed to generate a genus-specific region of the major outer membrane protein (MOMP) gene was used in a PCR to amplify a single DNA fragment of 245-259 bp. In the PCR, the expected single DNA fragment was amplified from strains of Chlamydia trachomatis, C. psittaci, C. pnenmoniae and C. pecorum, respectively. By restriction endonuclease analysis with AluI and PvuII, the amplified products exhibited four distinct patterns, corresponding to the four species. It is, therefore, concluded that one-step PCR followed by restriction endonuclease analysis as described in this study could be a valuable method for the detection and differentiation of Chlamydia species.
引用
收藏
页码:411 / 414
页数:4
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