CdS nanocrystal-based electrochemiluminescence biosensor for the detection of low-density lipoprotein by increasing sensitivity with gold nanoparticle amplification

被引:316
作者
Jie, Guifen [1 ]
Liu, Bo [1 ]
Pan, Hongcheng [1 ]
Zhu, Jun-Jie [1 ]
Chen, Hong-Yuan [1 ]
机构
[1] Nanjing Univ, Sch Chem & Chem Engn, Minist Educ, Key Lab Analyt Chem Life Sci, Nanjing 210093, Peoples R China
关键词
D O I
10.1021/ac062357c
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Mercaptoacetic acid (RSH)-capped CdS nanocrystals (NCs) was demonstrated to be electrochemically reduced during potential scan and react with the coreactant S2O82- to generate strong electrochemiluminescence (ECL) in aqueous solution. Based on the ECL of CdS NCs, a novel label-free ECL biosensor for the detection of low-density lipoprotein (LDL) has been developed by using self-assembly and gold nanoparticle amplification techniques. The biosensor was prepared as follows: The gold nanoparticles were first assembled onto a cysteamine monolayer on the gold electrode surface. This gold nanoparticle-covered electrode was next treated with cysteine and then reacted with CdS NCs to afford a CdS NC-electrode. Finally, apoB-100 (ligand of LDL receptor) was covalently conjugated to the CdS NC-electrode. The modification procedure was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, and atomic force microscopy, respectively. The resulting modified electrode was tested as ECL biosensor for LDL detection. The LDL concentration was measured through the decrease in ECL intensity resulting from the specific binding of LDL to apoB-100. The ECL peak intensity of the biosensor decreased linearly with LDL concentration in the range of 0.025-16 ng mL(-1) with a detection limit of 0.006 ng mL(-1). The CdS NCs not only showed high ECL intensity and good biocompatibility but also could provide more binding sites for apoB-100 loading. In addition, the gold nanoparticle amplification for protein ECL analysis was applied to the improvement of the detection sensitivity. Thus, the biosensor exhibited high sensitivity, good reproducibility, rapid response, and long-term stability.
引用
收藏
页码:5574 / 5581
页数:8
相关论文
共 47 条
  • [1] Electrochemistry and electrogenerated chemiluminescence of CdTe nanoparticles
    Bae, Y
    Myung, N
    Bard, AJ
    [J]. NANO LETTERS, 2004, 4 (06) : 1153 - 1161
  • [2] Dendrimer-modified silicon oxide surfaces as platforms for the deposition of gold and silver colloid monolayers: Preparation method, characterization, and correlation between microstructure and optical properties
    Bar, G
    Rubin, S
    Cutts, RW
    Taylor, TN
    Zawodzinski, TA
    [J]. LANGMUIR, 1996, 12 (05) : 1172 - 1179
  • [3] Morphology-dependent electrochemistry of cytochrome c at Au colloid-modified SnO2 electrodes
    Brown, KR
    Fox, AP
    Natan, MJ
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (05) : 1154 - 1157
  • [4] Semiconductor nanocrystals as fluorescent biological labels
    Bruchez, M
    Moronne, M
    Gin, P
    Weiss, S
    Alivisatos, AP
    [J]. SCIENCE, 1998, 281 (5385) : 2013 - 2016
  • [5] Nanoscale probes encapsulated by biologically localized embedding (PEBBLEs) for ion sensing and imaging in live cells
    Buck, SM
    Xu, H
    Brasuel, M
    Philbert, MA
    Kopelman, R
    [J]. TALANTA, 2004, 63 (01) : 41 - 59
  • [6] Quantum dot bioconjugates for ultrasensitive nonisotopic detection
    Chan, WCW
    Nie, SM
    [J]. SCIENCE, 1998, 281 (5385) : 2016 - 2018
  • [7] Alkanethiolate-protected PbS nanoclusters: Synthesis, spectroscopic and electrochemical studies
    Chen, SW
    Truax, LA
    Sommers, JM
    [J]. CHEMISTRY OF MATERIALS, 2000, 12 (12) : 3864 - 3870
  • [8] Flow injection analysis system equipped with a newly designed electrochemiluminescent detector and its application for detection of 2-thiouracil
    Chi, YW
    Duan, JP
    Lin, SD
    Chen, GN
    [J]. ANALYTICAL CHEMISTRY, 2006, 78 (05) : 1568 - 1573
  • [9] A microchip-based multianalyte assay system for the assessment of cardiac risk
    Christodoulides, N
    Tran, M
    Floriano, PN
    Rodriguez, M
    Goodey, A
    Ali, M
    Neikirk, D
    McDevitt, JT
    [J]. ANALYTICAL CHEMISTRY, 2002, 74 (13) : 3030 - 3036
  • [10] Multichannel electrochemiluminescence of luminol in neutral and alkaline aqueous solutions on a gold nanoparticle self-assembled electrode
    Cui, H
    Xu, Y
    Zhang, ZF
    [J]. ANALYTICAL CHEMISTRY, 2004, 76 (14) : 4002 - 4010