Nitric oxide induces chemotaxis of neutrophil-like HL-60 cells and translocation of cofilin to plasma membranes

被引:25
作者
Adachi, R
Matsui, S
Kinoshita, M
Nagaishi, K
Sasaki, H
Kasahara, T
Suzuki, K
机构
[1] Natl Inst Hlth Sci, Div Xenobiot Metab & Disposit, Setagaya Ku, Tokyo 1588501, Japan
[2] Kyoritsu Coll Pharmaceut Sci, Minato Ku, Tokyo 1058512, Japan
来源
INTERNATIONAL JOURNAL OF IMMUNOPHARMACOLOGY | 2000年 / 22卷 / 11期
关键词
nitric oxide; cofilin; chemotaxis; neutrophils; phagocytes;
D O I
10.1016/S0192-0561(00)00045-X
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Nitric oxide (NO) plays various important roles in the physiological system. With regard to chemotaxis of neutrophils, there are reports that endogenous NO is a mediator of chemotaxis, and others that exogenous NO inhibits chemotaxis. It is also reported that NO itself expressed chemotactic activity. On the other hand, we have recently proposed the importance of cofilin, an actin-binding phosphoprotein, in phagocyte functions through dephosphorylation and translocation to the plasma membrane regions. Because chemotaxis is a phenomenon of dynamic cell movement, cofilin, a regulator of the cytoskeletal system, may be involved in its mechanisms. To clarify further the effect of NO on functions of leukocytes and to examine the effect of NO on cofilin, we investigated the chemotaxis of neutrophil-like HL-60 cells induced by NO, as well as the influence of NO on the phosphorylation and intracellular distribution of cofilin. Two NO donors, 3-[2-hydroxy-1-(1-methylethyl)-2-nitrosohydrazino]-1-propanamine (NOC5) and S-nitroso-N-acetylpenicillamine (SNAP), were shown to cause chemotaxis, and, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazole 3-oxide (carboxy-PTIO), a NO-specific scavenger, inhibited the chemotaxis induced by NO-donors, suggesting that NO itself released from the NO donors has chemotactic activity. LY-83583 and 1H-[1,2,4]oxadiazolo[4,3-a]quinoxaline-1-one (ODQ), inhibitors of soluble guanylate cyclase, inhibited the chemotaxis to NO donors, which implies that soluble guanylate cyclase is involved in the signaling pathway of this NO action. We also found that NO caused translocation of cofilin to the cell periphery, though dephosphorylation of cofilin was not detected. These results demonstrate that NO has chemotactic activity for neutrophils and caused the translocation of cofilin to the plasma membrane regions without its dephosphorylation. (C) 2000 International Society for Immunopharmacology. Published by Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:855 / 864
页数:10
相关论文
共 54 条
[1]  
Aizawa H, 1997, J CELL SCI, V110, P2333
[2]   ANTAGONISTIC ACTION OF IMIDAZOLINEOXYL N-OXIDES AGAINST ENDOTHELIUM-DERIVED RELAXING FACTOR .NO THROUGH A RADICAL REACTION [J].
AKAIKE, T ;
YOSHIDA, M ;
MIYAMOTO, Y ;
SATO, K ;
KOHNO, M ;
SASAMOTO, K ;
MIYAZAKI, K ;
UEDA, S ;
MAEDA, H .
BIOCHEMISTRY, 1993, 32 (03) :827-832
[3]  
Andonegui G, 1999, J IMMUNOL, V162, P2922
[4]   Regulation of actin dynamics through phosphorylation of cofilin by LIM-kinase [J].
Arber, S ;
Barbayannis, FA ;
Hanser, H ;
Schneider, C ;
Stanyon, CA ;
Bernard, O ;
Caroni, P .
NATURE, 1998, 393 (6687) :805-809
[5]   Putting a new twist on actin: ADF/cofilins modulate actin dynamics [J].
Bamburg, JR ;
McGough, A ;
Ono, S .
TRENDS IN CELL BIOLOGY, 1999, 9 (09) :364-370
[6]   EXOGENOUS NITRIC-OXIDE ELICITS CHEMOTAXIS OF NEUTROPHILS IN-VITRO [J].
BEAUVAIS, F ;
MICHEL, L ;
DUBERTRET, L .
JOURNAL OF CELLULAR PHYSIOLOGY, 1995, 165 (03) :610-614
[7]  
BELENKY SN, 1993, J LAB CLIN MED, V122, P388
[8]   NITRIC-OXIDE - A PHYSIOLOGICAL MESSENGER MOLECULE [J].
BREDT, DS ;
SNYDER, SH .
ANNUAL REVIEW OF BIOCHEMISTRY, 1994, 63 :175-195
[9]   NITRIC-OXIDE STIMULATES ADP-RIBOSYLATION OF ACTIN IN ASSOCIATION WITH THE INHIBITION OF ACTIN POLYMERIZATION IN HUMAN NEUTROPHILS [J].
CLANCY, R ;
LESZCZYNSKA, J ;
AMIN, A ;
LEVARTOVSKY, D ;
ABRAMSON, SB .
JOURNAL OF LEUKOCYTE BIOLOGY, 1995, 58 (02) :196-202
[10]   TERMINAL DIFFERENTIATION OF HUMAN PROMYELOCYTIC LEUKEMIA-CELLS INDUCED BY DIMETHYL-SULFOXIDE AND OTHER POLAR COMPOUNDS [J].
COLLINS, SJ ;
RUSCETTI, FW ;
GALLAGHER, RE ;
GALLO, RC .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (05) :2458-2462