Use of Rotofor preparative isoelectrofocusing cell in protein purification procedure

被引：28

作者：

Ayala, A ^{[1
]}

Parrado, J ^{[1
]}

Machado, A ^{[1
]}

机构：

[1] Univ Sevilla, Fac Farm, Dept Bioquim Bromatol & Toxicol, E-41012 Seville, Spain

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1998年 / 69卷 / 01期

关键词：

Rotofor; superoxide dismutase; 6-phosphogluconate dehydrogenase;

D O I：

10.1007/BF02786017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Rotofor cell is a preparative isoelectric focusing (IEF) apparatus, in which IEF is performed entirely in free solution. Electrofocusing in Rotofor cell has been described as well-suited for use at any stage of a purification scheme. However, it has some important limitations in resolving complex mixtures of proteins. This paper describes the advantages and disadvantages of using the Rotofor cell in purification protocols.

引用

页码：11 / 16

页数：6

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