Molecular dissection of the human B-cell response against Toxoplasma gondii infection by lambda display of cDNA libraries

被引：51

作者：

Beghetto, E

Spadoni, A

Buffolano, W

Del Pezzo, M

Minenkova, O

Pavoni, E

Pucci, A

Cortese, R

Felici, F

Gargano, N

机构：

[1] Kenton Labs, I-00040 Monte Porzio Catone, Italy

[2] Univ Naples Federico II, Dept Pediat, Naples, Italy

[3] Ist Ric Biol Mol P Angeletti, I-00040 Pomezia, Italy

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 2003年 / 33卷 / 02期

关键词：

phage display; cDNA library; Toxoplasma gondii;

D O I：

10.1016/S0020-7519(02)00256-4

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The disorders generated by Toxoplasma gondii infection are closely associated with the competence of the host immune system and both humoral and cell mediated immunity are involved in response to parasite invasion. To identify antigens implicated in human B-cell responses, we screened a phage-display library of T. gondii cDNA fragments with sera of infected individuals. This approach identified a panel of recombinant phage clones carrying B-cell epitopes. All the peptide sequences selected by this procedure are regions of T. gondii gene products. These regions-contain epitopes of the T. gondii antigens SAG I, GRA1, GRA7, GRA8 and MIC5, which are recognised by human immunoglobulins. Moreover, we report the isolation and characterisation of two additional immunodominant regions encoded by GRA3 and MIC3 genes, whose products have never been described as antigens of the human B-cell response against T. gondii infection. These results demonstrate potential of lambda-display technology for antigen discovery and for the study of the human antibody response against infectious agents. (C) 2002 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.

引用

页码：163 / 173

页数：11

共 40 条

[1] Identification of a human immunodominant B-cell epitope within the GRA1 antigen of Toxoplasma gondii by phage display of cDNA libraries [J].