Multiplexed protein detection by proximity ligation for cancer biomarker validation

被引：153

作者：

Fredriksson, Simon

Dixon, William

Ji, Hanlee

Koong, Albert C.

Mindrinos, Michael

Davis, Ronald W.

机构：

[1] Stanford Genome Technol Ctr, BioX, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Radiat Oncol, Stanford, CA 94305 USA

来源：

NATURE METHODS | 2007年 / 4卷 / 04期

关键词：

D O I：

10.1038/NMETH1020

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a proximity ligation-based multiplexed protein detection procedure in which several selected proteins can be detected via unique nucleic-acid identifiers and subsequently quantified by real-time PCR. The assay requires a 1-mu l sample, has low-femtomolar sensitivity as well as five-log linear range and allows for modular multiplexing without cross-reactivity. The procedure can use a single polyclonal antibody batch for each target protein, simplifying affinity-reagent creation for new biomarker candidates.

引用

页码：327 / 329

页数：3

共 15 条

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