Metabolically engineered methylotrophic yeast cells and enzymes as sensor biorecognition elements

被引：10

作者：

Gonchar, M

Maidan, M

Korpan, Y

Sibirny, V

Kotylak, Z

Sibirny, A

机构：

[1] Inst Cell Biol, Dept Mol Genet & Biotechnol, UA-79005 Lvov, Ukraine

[2] Inst Cell Biol, Dept Cell Regulatory Syst, UA-79005 Lvov, Ukraine

[3] Inst Mol Biol & Genet, Dept Translat Mech Genet Informat, UA-03143 Kiev, Ukraine

[4] Politechnika Swietokrzyska, PL-25314 Kielce, Poland

[5] Univ Rzeszow, PL-35310 Rzeszow, Poland

来源：

FEMS YEAST RESEARCH | 2002年 / 2卷 / 03期

关键词：

yeast; metabolic engineering; biorecognition element; biosensor;

D O I：

10.1016/S1567-1356(02)00124-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An extended definition of the term metabolic engineering is given and its successful use in the construction of biorecognition elements of sensors is demonstrated. It is shown that genetic and chemical modifications of methylotrophic yeast cells provide directed changes in their physiological responses towards methanol, ethanol and formaldehyde resulting in enhanced selectivity and shorter time response of the corresponding potentiometric and amperometric biosensors. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：307 / 314

页数：8

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