Sequence effects of single base loops in intramolecular quadruplex DNA

被引：120

作者：

Rachwal, Phillip A.

Brown, Tom

Fox, Keith R.

机构：

[1] Univ Southampton, Sch Biol Sci, Southampton SO16 7PX, Hants, England

[2] Univ Southampton, Sch Chem, Southampton SO17 1BJ, Hants, England

来源：

FEBS LETTERS | 2007年 / 581卷 / 08期

基金：

英国生物技术与生命科学研究理事会;

关键词：

DNA quadruplex; DNA structure;

D O I：

10.1016/j.febslet.2007.03.040

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have examined the properties of intramolecular G-quadruplexes in which the G3 tracts are separated by single base loops. The most stable complex contained 1',2'-dideoxyribose in all three loops, while loops containing T and C were slightly less stable (by about 2 degrees C). Quadruplexes containing loops with single A residues were less stable by 8 degrees C for each T to A substitution. These folded sequences display similar CD spectra, which are consistent with the formation of parallel stranded complexes with double-chain reversal loops. These results demonstrate that loop sequence, and not just length, affects quadruplex stability. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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收藏

页码：1657 / 1660

页数：4

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