Echinococcus granulosus:: DNA extraction from germinal layers allows strain determination in fertile and nonfertile hydatid cysts

被引：46

作者：

Kamenetzky, L ^{[1
]}

Canova, SG ^{[1
]}

Guarnera, EA ^{[1
]}

Rosenzvit, MC ^{[1
]}

机构：

[1] Dept Parasitol Sanitaria, Inst Nacl Parasitol, Adm Nacl Lab & Inst Salud Dr Carlos G Malbran, RA-1281 Buenos Aires, DF, Argentina

来源：

EXPERIMENTAL PARASITOLOGY | 2000年 / 95卷 / 02期

关键词：

Echinococcus granulosus; Cestoda; parasite; genetic variation; hydatid layers; nonfertile hydatid cysts; diatomaceous earth;

D O I：

10.1006/expr.2000.4518

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A method for the isolation of Echinococcus granulosus DNA from germinal layers of hydatid cysts is described. The method includes a hexadecyltrimethylammonium bromide/chloroform extraction and an adsorption to diatomaceous earth suspension. DNA suitable for polymerase chain reaction was obtained and used for parasite strain determination by mirochondrial cytochrome c oxidase I gene sequencing. Fertile and nonfertile cyst isolates from sheep, cattle, pigs, and humans were characterized. Hitherto, no direct parasite strain characterization has bt rn made on nonfertile hydatid cysts, whereas here we report that nonfertile hydatid cysts were produced by sheep strain (G1 genotype) in sheep, cattle, and humans and by pig strain (G7 genotype) in pigs. (C) 2000 Academic Press.

引用

页码：122 / 127

页数：6

共 20 条

[1] RAPID AND SIMPLE METHOD FOR PURIFICATION OF NUCLEIC-ACIDS [J].

BOOM, R ;

SOL, CJA ;

SALIMANS, MMM ;

JANSEN, CL ;

WERTHEIMVANDILLEN, PME ;

VANDERNOORDAA, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (03) :495-503

[2] ULTRASTRUCTURAL ASPECTS OF FERTILE AND STERILE CYSTS OF ECHINOCOCCUS-GRANULOSUS DEVELOPED IN HOSTS OF DIFFERENT SPECIES [J].

BORTOLETTI, G ;

FERRETTI, G .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1978, 8 (06) :421-431

[3] MOLECULAR-GENETIC CHARACTERIZATION OF THE CERVID STRAIN (NORTHERN FORM) OF ECHINOCOCCUS-GRANULOSUS [J].

BOWLES, J ;

BLAIR, D ;

MCMANUS, DP .

PARASITOLOGY, 1994, 109 :215-221

[4] NADH DEHYDROGENASE-1 GENE-SEQUENCES COMPARED FOR SPECIES AND STRAINS OF THE GENUS ECHINOCOCCUS [J].

BOWLES, J ;

MCMANUS, DP .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1993, 23 (07) :969-972

[5] GENETIC-VARIANTS WITHIN THE GENUS ECHINOCOCCUS IDENTIFIED BY MITOCHONDRIAL-DNA SEQUENCING [J].

BOWLES, J ;

BLAIR, D ;

MCMANUS, DP .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1992, 54 (02) :165-174

[6] FURTHER EVIDENCE FOR THE OCCURRENCE OF A DISTINCT STRAIN OF ECHINOCOCCUS-GRANULOSUS IN EUROPEAN PIGS [J].

ECKERT, J ;

THOMPSON, RCA ;

LYMBERY, AJ ;

PAWLOWSKI, ZS ;

GOTTSTEIN, B ;

MORGAN, UM .

PARASITOLOGY RESEARCH, 1993, 79 (01) :42-48

[7] STUDIES ON POLYSACCHARIDES OF ECHINOCOCCUS-GRANULOSUS CYST, WITH OBSERVATIONS ON A POSSIBLE MECHANISM FOR LAMINATED MEMBRANE FORMATION [J].

KILEJIAN, A ;

SCHWABE, CW .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY, 1971, 40 (1B) :25-+

[8] FINE-STRUCTURE OF GERMINAL MEMBRANE OF ECHINOCOCCUS-GRANULOSUS CYSTS [J].

LASCANO, EF ;

COLTORTI, EA ;

VARELADIAZ, VM .

JOURNAL OF PARASITOLOGY, 1975, 61 (05) :853-860

[9] GENETIC-DIFFERENCES BETWEEN CYSTS OF ECHINOCOCCUS-GRANULOSUS FROM THE SAME HOST [J].

LYMBERY, AJ ;

THOMPSON, RCA .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1989, 19 (08) :961-964

[10] PHYLOGENETIC-RELATIONSHIPS WITHIN TAENIA-TAENIAEFORMIS VARIANTS AND OTHER TAENIID CESTODES INFERRED FROM THE NUCLEOTIDE-SEQUENCE OF THE CYTOCHROME-C-OXIDASE SUBUNIT-I GENE [J].

OKAMOTO, M ;

BESSHO, Y ;

KAMIYA, M ;

KUROSAWA, T ;

HORII, T .

PARASITOLOGY RESEARCH, 1995, 81 (06) :451-458

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