A Sec14p-nodulin domain phosphatidylinositol transfer protein polarizes membrane growth of Arabidopsis thaliana root hairs

被引:162
作者
Vincent, P [1 ]
Chua, M
Nogue, F
Fairbrother, A
Mekeel, H
Xu, Y
Allen, N
Bibikova, TN
Gilroy, S
Bankaitis, VA
机构
[1] Univ N Carolina, Sch Med, Michael Hooker Microscopy Facil, Dept Cell & Dev Biol, Chapel Hill, NC 27599 USA
[2] Univ N Carolina, Sch Med, Michael Hooker Microscopy Facil, Dept Cell & Mol Physiol, Chapel Hill, NC 27599 USA
[3] INRA, Genet & Ameliorat Plantes Stn, F-78026 Versailles, France
[4] N Carolina State Univ, Dept Bot, Raleigh, NC 27695 USA
[5] Penn State Univ, Dept Biol, University Pk, PA 16802 USA
关键词
D O I
10.1083/jcb.200412074
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Phosphatidylinositol (Ptdlns) transfer proteins (PITPs) regulate signaling interfaces between lipid metabolism and membrane trafficking. Herein, we demonstrate that AtSfh1p, a member of a large and uncharacterized Arabidopsis thaliana Secl4p-nodulin domain family, is a PITP that regulates a specific stage in root hair development. AtSfh1p localizes along the root hair plasma membrane and is enriched in discrete plasma membrane domains and in the root hair tip cytoplasm. This localization pattern recapitulates that visualized for Ptdlns(4,5)P-2 in developing root hairs. Gene ablation experiments show AtSfh1p nullizygosity compromises polarized root hair expansion in a manner that coincides with loss of tip-directed Ptdlns(4,5)P2, dispersal of secretary vesicles from the tip cytoplasm, loss of the tip f-actin network, and manifest disorganization of the root hair microtubule cytoskeleton. Derangement of tip-directed Ca2+ gradients is also apparent and results from isotropic influx of Ca2+ from the extracellular milieu. We propose AtSfh1p regulates intracellular and plasma membrane phosphoinositide polarity landmarks that focus membrane trafficking, Ca2+ signaling, and cytoskeleton functions to the growing root hair apex. We further suggest that Sec14p-nodulin domain proteins represent a family of regulators of polarized membrane growth in plants.
引用
收藏
页码:801 / 812
页数:12
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