Multiple subphases of DNA replication in Chinese hamster ovary (CHO-K1) cells

被引：10

作者：

Banfalvi, G

Mikhailova, M

Poirier, LA

Chou, MW

机构：

[1] Semmelweis Univ Med, Sch Med, Dept Med Chem Mol Biol & Pathobiochema, H-1088 Budapest, Hungary

[2] Natl Ctr Toxicol Res, Div Biochem Toxicol, Jefferson, AR 72079 USA

[3] Natl Ctr Toxicol Res, Div Mol Epidemiol, Jefferson, AR 72079 USA

来源：

DNA AND CELL BIOLOGY | 1997年 / 16卷 / 12期

关键词：

D O I：

10.1089/dna.1997.16.1493

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Replicative DNA synthesis has been measured throughout the S phase in synchronized populations of Chinese hamster ovary cells, When exponentially growing, cells in suspension cultures were subjected to counterflow centrifugal elutriation and the resolution power was increased the biphasic replication profile has been resolved and multiple subphases were distinguished, These replication peaks, termed replication checkpoints, are distributed evenly throughout the S phase, The replication checkpoints have been characterized by their average C values corresponding to 2.05, 2.12, 2.2, 2.45, 2.6, 2.8, 2.95, 3.15, 3.3, 3.45, and 3.85.

引用

页码：1493 / 1498

页数：6

共 15 条

[1] THE SAD1/RAD53 PROTEIN-KINASE CONTROLS MULTIPLE CHECKPOINTS AND DNA DAMAGE-INDUCED TRANSCRIPTION IN YEAST [J].