Stat3 Is Required for Anchorage-Independent Growth and Metastasis But Not for Mammary Tumor Development Downstream of the ErbB-2 Oncogene

被引：27

作者：

Barbieri, Isaia ^{[1
,2
]}

Quaglino, Elena ^{[1
,3
]}

Maritano, Diego ^{[2
]}

Pannellini, Tania ^{[4
]}

Riera, Ludovica ^{[5
]}

Cavallo, Federica ^{[1
,3
]}

Forni, Guido ^{[1
,3
]}

Musiani, Piero ^{[4
]}

Chiarle, Roberto ^{[5
]}

Poli, Valeria ^{[1
,2
]}

机构：

[1] Univ Turin, Ctr Mol Biotechnol, I-10126 Turin, Italy

[2] Univ Turin, Dept Genet Biol & Biochem, I-10126 Turin, Italy

[3] Univ Turin, Dept Clin & Biol Sci, I-10126 Turin, Italy

[4] Univ G dAnnunzio, Ageing Res Ctr CeSI, Chieti, Italy

[5] San Giovanni Battista Hosp, CERMS, Turin, Italy

来源：

MOLECULAR CARCINOGENESIS | 2010年 / 49卷 / 02期

关键词：

neu; conditional mutagenesis; breast tumors; transgenic mice; TRANSGENIC MICE; BREAST-CANCER; EPITHELIAL-CELLS; ACTIVATION; EXPRESSION; CARCINOGENESIS; KINASE; TARGET; LINES; ADENOCARCINOMAS;

D O I：

10.1002/mc.20605

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The oncogenic transcription factor Stat3 is constitutively active in a high percentage of human tumors including mammary adenocarcinomas and is reported to participate in the ErbB-2 oncogene signaling. In order to assess the role of signal transducer and activator of transcription 3 (Stat3) in mammary tumorigenesis downstream of ErbB-2, we generated mice expressing the activated rat ErbB-2 (neu) but lacking Stat3 in the mammary epithelium. Stat3 is apparently not required for neu-driven mammary tumorigenesis as tumors developed similarly in both Stat3-sufficient and Stat3-deficient glands. However, short hairpin RNA (shRNA)-mediated Stat3 silencing in a neu-overexpressing tumor-derived cell line completely abolished both neu-driven anchorage-independent growth and lung metastasis. Our data suggest that Stat3 might be a useful therapeutic target in breast tumors showing amplification and/or overexpression of the ErbB-2 oncogene, which normally display aggressive, metastatic behavior. (C) 2009 Wiley-Liss, Inc.

引用

页码：114 / 120

页数：7

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