The role of modifications in codon discrimination by tRNALys UUU

被引：279

作者：

Murphy, FV

Ramakrishnan, V

Malkiewicz, A

Agris, PF

机构：

[1] MRC, Mol Biol Lab, Cambridge CB2 2QH, England

[2] Tech Univ Lodz, Inst Organ Chem, PL-90924 Lodz, Poland

[3] N Carolina State Univ, Dept Mol & Struct Biochem, Raleigh, NC 27695 USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2004年 / 11卷 / 12期

关键词：

D O I：

10.1038/nsmb861

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The natural modification of specific nucleosides in many tRNAs is essential during decoding of mRNA by the ribosome. For example, tRNA(UUU)(Lys) requires the modification N6-threonylcarbamoyladenosine at position 37 (t(6)A37), adjacent and 3 to the anticodon, to bind AAA in the A site of the ribosomal 30S subunit. Moreover, it can only bind both AAA and AAG lysine codons when doubly modified with t(6)A37 and either 5-methylaminomethyluridine or 2-thiouridine at the wobble position (mnm(5)U34 or s(2)U34). Here we report crystal structures of modified tRNA anticodon stem-loops bound to the 30S ribosomal subunit with lysine codons in the A site. These structures allow the rationalization of how modifications in the anticodon loop enable decoding of both lysine codons AAA and AAG.

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页码：1186 / 1191

页数：6

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