Identification of a testicular odorant receptor mediating human sperm chemotaxis

被引:562
作者
Spehr, M
Gisselmann, G
Poplawski, A
Riffell, JA
Wetzel, CH
Zimmer, RK
Hatt, H
机构
[1] Ruhr Univ Bochum, Dept Cell Physiol, D-44780 Bochum, Germany
[2] Univ Calif Los Angeles, Program Neurosci, Los Angeles, CA 90095 USA
[3] Univ Calif Los Angeles, Dept Biol, Los Angeles, CA 90095 USA
[4] Univ Calif Los Angeles, Brain Res Inst, Los Angeles, CA 90095 USA
关键词
D O I
10.1126/science.1080376
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Although it has been known for some time that olfactory receptors (ORs) reside in spermatozoa, the function of these ORs is unknown. Here, we identified, cloned, and functionally expressed a previously undescribed human testicular OR, hOR17-4. With the use of raticifluorometric imaging, Ca2+ signals were induced by a small subset of applied chemical stimuli, establishing the molecular receptive fields for the recombinantly expressed receptor in human embryonic kidney (HEK) 293 cells and the native receptor in human spermatozoa. Bourgeonal was a powerful agonist for both recombinant and native receptor types, as well as a strong chemoattractant in subsequent behavioral bioassays. In contrast, undecanal was a potent OR antagonist to bourgeonal and related compounds. Taken together, these results indicate that hOR17-4 functions in human sperm chemotaxis and may be a critical component of the fertilization process.
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收藏
页码:2054 / 2058
页数:5
相关论文
共 20 条
[1]   Signal transduction pathways in human spermatozoa [J].
Baldi, E ;
Luconi, M ;
Bonaccorsi, L ;
Forti, G .
JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 2002, 53 (1-2) :121-131
[2]   OLFACTORY RECEPTOR GENE-CLUSTER ON HUMAN-CHROMOSOME-17 - POSSIBLE DUPLICATION OF AN ANCESTRAL RECEPTOR REPERTOIRE [J].
BENARIE, N ;
LANCET, D ;
TAYLOR, C ;
KHEN, M ;
WALKER, N ;
LEDBETTER, DH ;
CARROZZO, R ;
PATEL, K ;
SHEER, D ;
LEHRACH, H ;
NORTH, MA .
HUMAN MOLECULAR GENETICS, 1994, 3 (02) :229-235
[3]  
Eisenbach M, 1999, BIOESSAYS, V21, P203, DOI 10.1002/(SICI)1521-1878(199903)21:3<203::AID-BIES4>3.0.CO
[4]  
2-T
[5]   How the olfactory system makes sense of scents [J].
Firestein, S .
NATURE, 2001, 413 (6852) :211-218
[6]   Progesterone-mediated calcium influx and acrosome reaction of human spermatozoa: Pharmacological investigation of T-type calcium channels [J].
Garcia, MA ;
Meizel, S .
BIOLOGY OF REPRODUCTION, 1999, 60 (01) :102-109
[7]   The olfactory adenylyl cyclase III is expressed in rat germ cells during spermiogenesis [J].
Gautier-Courteille, C ;
Salanova, M ;
Conti, M .
ENDOCRINOLOGY, 1998, 139 (05) :2588-2599
[8]   The olfactory receptor gene superfamily: data mining, classification, and nomenclature [J].
Glusman, G ;
Bahar, A ;
Sharon, D ;
Pilpel, Y ;
White, J ;
Lancet, D .
MAMMALIAN GENOME, 2000, 11 (11) :1016-1023
[9]   The complete human olfactory subgenome [J].
Glusman, G ;
Yanai, I ;
Rubin, I ;
Lancet, D .
GENOME RESEARCH, 2001, 11 (05) :685-702
[10]   Identification and functional analysis of splice variants of the germ cell soluble adenylyl cyclase [J].
Jaiswal, BS ;
Conti, M .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (34) :31698-31708