Survey for the presence of specific free-living amoebae in cooling waters from Belgian power plants

被引：47

作者：

Behets, Jonas

Declerck, Priscilla

Delaedt, Yasmine

Verelst, Lieve

Ollevier, Frans

机构：

[1] Katholieke Univ Leuven, Aquat Ecol Lab, B-3000 Louvain, Belgium

[2] LABORELEC, B-1630 Linkebeek, Belgium

来源：

PARASITOLOGY RESEARCH | 2007年 / 100卷 / 06期

关键词：

D O I：

10.1007/s00436-006-0399-1

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Free-living amoebae (FLA) are distributed ubiquitously in aquatic environments with increasing importance in hygienic, medical and ecological relationships to man. In this study, water samples from Belgian industrial cooling circuits were quantitatively surveyed for the presence of FLA. Isolated, thermotolerant amoebae were identified morphologically as well as using the following molecular methods: enzyme-linked immunosorbent assay and isoenzyme electrophoresis and PCR. Thermophilic amoebae were present at nearly all collection sites, and the different detection methods gave similar results. Naegleria fowleri was the most frequently encountered thermotolerant species, and concentrations of thermotolerant FLA were correlated with higher temperatures.

引用

页码：1249 / 1256

页数：8

共 26 条

[1] A molecular approach to the characterization of the eukaryotic communities of an extreme acidic environment: Methods for DNA extraction and denaturing gradient gel electrophoresis analysis [J].