Gene expression of inflammatory mediators in different chambers of the human heart

被引:17
作者
Valen, G
Paulsson, G
Bennet, AM
Hansson, GK
Vaage, J
机构
[1] Karolinska Hosp, Dept Thorac Surg, Ctr Mol Med, S-17176 Stockholm, Sweden
[2] Karolinska Hosp, Cardiovasc Res Unit, Ctr Mol Med, S-17176 Stockholm, Sweden
关键词
D O I
10.1016/S0003-4975(00)01507-1
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background. Inflammatory genes may be unevenly expressed in different heart chambers. Methods. Biopsies were taken simultaneously from the right atrium (RA), left atrium (LA), and left ventricle (LV) of 19 patients before cardioplegic arrest during open heart surgery. The mRNA expression of tumor necrosis factor alpha (TNF alpha), interleukin 1 beta (IL-1 beta), inducible and endothelial nitric oxide synthase (iNOS and eNOS), endothelin-l (ET-1), E-selectin (CD62E), intercellular adhesion molecule-1 (ICAM-1) and its ligand CD18, and CD25 was evaluated with semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). Results. Expression of TNF alpha mRNA was higher in RA than LA and LV (p < 0.05), whereas IL-1 beta was more expressed in LA than RA (p < 0.05), which was higher than LV (p < 0.0001). There were no significant regional differences in the expression of ICAM-1, CD62E, CD25, iNOS, and eNOS. CD18 was higher in RA than LA (p < 0.05); ET-1 was more expressed in RA than LV (p < 0.04). Patients with stable angina had no expression of eNOS. Conclusions. Gene expression of inflammatory mediators was detected in the hearts of patients with different cardiovascular disorders, and was unevenly distributed in different heart chambers. Cardiac biopsies should be taken from the same site. (C) 2000 by The Society of Thoracic Surgeons.
引用
收藏
页码:562 / 567
页数:6
相关论文
共 23 条
[1]   Ventilation and oxygenation induce endothelial nitric oxide synthase gene expression in the lungs of fetal lambs [J].
Black, SM ;
Johengen, MJ ;
Ma, ZD ;
Bristow, J ;
Soifer, SJ .
JOURNAL OF CLINICAL INVESTIGATION, 1997, 100 (06) :1448-1458
[2]   T-cell and monocyte subsets, inflammatory molecules, rejection, and hemodynamics early after cardiac transplantation [J].
Deng, MC ;
Erren, M ;
Roeder, N ;
Dreimann, V ;
Günther, F ;
Kerber, S ;
Baba, HA ;
Schmidt, C ;
Breithardt, G ;
Scheld, HH .
TRANSPLANTATION, 1998, 65 (09) :1255-1261
[3]  
Devaux B, 1997, EUR HEART J, V18, P470
[4]   Interleukin-1 in myocardium and coronary arteries of patients with dilated cardiomyopathy [J].
Francis, SE ;
Holden, H ;
Holt, CM ;
Duff, GW .
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 1998, 30 (02) :215-223
[5]   Expression of endothelin-1 and endothelin-converting enzyme-1 mRNAs and proteins in failing human hearts [J].
Fukuchi, M ;
Giaid, A .
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 1998, 31 :S421-S423
[6]   Heterogeneous expression and activity of endothelial and inducible nitric oxide synthases in end-stage human heart failure -: Their relation to lesion site and β-adrenergic receptor therapy [J].
Fukuchi, M ;
Hussain, SNA ;
Giaid, A .
CIRCULATION, 1998, 98 (02) :132-139
[7]   QUANTITATIVE POLYMERASE CHAIN-REACTION ANALYSIS OF MDR1 MESSENGER-RNA IN MULTIPLE-MYELOMA CELL-LINES AND CLINICAL SPECIMENS [J].
FUTSCHER, BW ;
BLAKE, LL ;
GERLACH, JH ;
GROGAN, TM ;
DALTON, WS .
ANALYTICAL BIOCHEMISTRY, 1993, 213 (02) :414-421
[8]   Cell-mediated immunity in atherosclerosis [J].
Hansson, GK .
CURRENT OPINION IN LIPIDOLOGY, 1997, 8 (05) :301-311
[9]   Expression of inducible nitric oxide synthase fin human heart failure [J].
Haywood, GA ;
Tsao, PS ;
vonderLeyen, HE ;
Mann, MJ ;
Kelling, PJ ;
Trindade, PT ;
Lewis, NP ;
Byrne, CD ;
Rickenbacher, PR ;
Bishopric, NH ;
Cooke, JP ;
McKenna, WJ ;
Fowler, MB .
CIRCULATION, 1996, 93 (06) :1087-1094
[10]   INDUCTION OF INTERCELLULAR-ADHESION MOLECULE-1 AND E-SELECTIN MESSENGER-RNA IN HEART AND SKELETAL-MUSCLE OF PEDIATRIC-PATIENTS UNDERGOING CARDIOPULMONARY BYPASS [J].
KILBRIDGE, PM ;
MAYER, JE ;
NEWBURGER, JW ;
HICKEY, PR ;
WALSH, AZ ;
NEUFELD, EJ .
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY, 1994, 107 (05) :1183-1192