Cloning, expression and evaluation of a recombinant sub-unit vaccine against Clostridium botulinum type F toxin

被引：34

作者：

Holley, JL ^{[1
]}

Elmore, M

Mauchline, M

Minton, N

Titball, RW

机构：

[1] Def Evaluat & Res Agcy, CBD Porton Down, Salisbury SP4 0JQ, Wilts, England

[2] Ctr Appl Microbiol & Res, Salisbury SP4 0JQ, Wilts, England

来源：

VACCINE | 2000年 / 19卷 / 2-3期

关键词：

FHc subunit; botulinum; vaccine;

D O I：

10.1016/S0264-410X(00)00155-9

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A synthetic gene encoding the He (binding) domain of Clostridium botulinum neurotoxin F (FHc) was expressed in Escherichia coli fused to maltose binding protein (MBP). The purified MBP-FHc and FHc isolated after removal of MBP were evaluated in mice for their ability to protect against toxin challenge. Balb/c mice developed a protective immune response following administration of either protein via the intraperitoneal or intramuscular routes. A comparison of antibody titres and protection following single and multiple vaccinations and the effects of dosage are shown. The long term protection afforded by the vaccines was also investigated. Ten months following vaccination mice were still protected when challenged with 10(4) MLD50 doses of botulinum toxin F. Crown Copyright (C) 2000 Published by Elsevier Science Ltd. All rights reserved.

引用

页码：288 / 297

页数：10

共 18 条

[1]

ARNON SS, 1991, CLOSTRIDIA MOL BIOL, P95

[2]

BROWN JE, 1991, CLOSTRIDIA MOL BIOL, P505

[3]

Buchanan R. E., 1984, Bergey's Manual of Determinative Bacteriology

[4] Purification, potency, and efficacy of the botulinum neurotoxin type A binding domain from Pichia pastoris as a recombinant vaccine candidate [J].

Byrne, MP ;

Smith, TJ ;

Montgomery, VA ;

Smith, LA .

INFECTION AND IMMUNITY, 1998, 66 (10) :4817-4822

[5] THE PMTL NIC-CLONING VECTORS .1. IMPROVED PUC POLYLINKER REGIONS TO FACILITATE THE USE OF SONICATED DNA FOR NUCLEOTIDE SEQUENCING [J].

CHAMBERS, SP ;

PRIOR, SE ;

BARSTOW, DA ;

MINTON, NP .

GENE, 1988, 68 (01) :139-149

[6] Antibody mapping to domains of botulinum neurotoxin serotype A in the complexed and uncomplexed forms [J].

Chen, F ;

Kuziemko, GM ;

Amersdorfer, P ;

Wong, C ;

Marks, JD ;

Stevens, RC .

INFECTION AND IMMUNITY, 1997, 65 (05) :1626-1630

[7] HIGH-LEVEL EXPRESSION OF TETANUS TOXIN FRAGMENT-C IN PICHIA-PASTORIS STRAINS CONTAINING MULTIPLE TANDEM INTEGRATIONS OF THE GENE [J].

CLARE, JJ ;

RAYMENT, FB ;

BALLANTINE, SP ;

SREEKRISHNA, K ;

ROMANOS, MA .

BIO-TECHNOLOGY, 1991, 9 (05) :455-460

[8] PROTECTIVE VACCINATION WITH A RECOMBINANT FRAGMENT OF CLOSTRIDIUM-BOTULINUM NEUROTOXIN SEROTYPE A EXPRESSED FROM A SYNTHETIC GENE IN ESCHERICHIA-COLI [J].

CLAYTON, MA ;

CLAYTON, JM ;

BROWN, DR ;

MIDDLEBROOK, JL .

INFECTION AND IMMUNITY, 1995, 63 (07) :2738-2742

[9]

CRITCHLEY EMR, 1990, BRIT J HOSP MED, V43, P290

[10] IMMUNIZATION OF MICE AGAINST TETANUS WITH FRAGMENTS OF TETANUS TOXIN SYNTHESIZED IN ESCHERICHIA-COLI [J].

FAIRWEATHER, NF ;

LYNESS, VA ;

MASKELL, DJ .

INFECTION AND IMMUNITY, 1987, 55 (11) :2541-2545

← 1 2 →