Disruption of α-mannosidase processing induces non-canonical hybrid-type glycosylation

被引：14

作者：

Crispin, Max

Aricescu, A. Radu

Chang, Veronica T.

Jones, E. Yvonne

Stuart, David I.

Dwek, Raymond A.

Davis, Simon J.

Harvey, David J.

机构：

[1] Univ Oxford, Div Struct Biol, Wellcome Trust Ctr Human Genet, Oxford OX3 7BN, England

[2] Univ Oxford, Nuffield Dept Clin Med, Oxford OX3 9DS, England

[3] John Radcliffe Hosp, MRC, Human Immunol Unit, Weatherall Inst Mol Med, Oxford OX3 9DS, England

[4] Univ Oxford, Dept Biochem, Glycobiol Inst, Oxford OX1 3QU, England

来源：

FEBS LETTERS | 2007年 / 581卷 / 10期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

hybrid-type glycosylation; GlcNAc transferase I; electrospray ionization mass spectrometry; swainsonine; golgi alpha-mannosidase II;

D O I：

10.1016/j.febslet.2007.04.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Golgi alpha-mannosidase 11 is essential for the efficient formation of complex-type glycosylation. Here, we demonstrate that the disruption of Golgi a-mannosidase 11 activity by swainsonine in human embryonic kidney cells is capable of inducing a novel class of hybrid-type glycosylation containing a partially processed mannose moiety. The discovery of 'Man(6)-based' hybrid-type glycans reveals a broader in vivo specificity of Nacetylglucosaminyltransferase 1, further defines the arm-specific tolerance of core alpha 1-6 fucosyltransferase to terminal a1-2 mannose residues, and suggests that disruption of Golgi a-mannosidase 11 activity is capable of inducing potentially 'non-self' structures. (C) 2007 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

收藏

页码：1963 / 1968

页数：6

相关论文

共 37 条

[1] A time- and cost-efficient system for high-level protein production in mammalian cells [J].

Aricescu, A. Radu ;

Lu, Weixian ;

Jones, E. Yvonne .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2006, 62 :1243-1250

[2] Molecular analysis of receptor protein tyrosine phosphatase μ-mediated cell adhesion [J].

Aricescu, AR ;

Hon, WC ;

Siebold, C ;

Lu, WX ;

van der Merwe, PA ;

Jones, EY .

EMBO JOURNAL, 2006, 25 (04) :701-712

[3] Receptor protein tyrosine phosphatases as mediators of cellular adhesion [J].

Beltran, PJ ;

Bixby, JL .

FRONTIERS IN BIOSCIENCE, 2003, 8 :D87-D99

[4] Antibody domain exchange is an immunological solution to carbohydrate cluster recognition [J].

Calarese, DA ;

Scanlan, CN ;

Zwick, MB ;

Deechongkit, S ;

Mimura, Y ;

Kunert, R ;

Zhu, P ;

Wormald, MR ;

Stanfield, RL ;

Roux, KH ;

Kelly, JW ;

Rudd, PM ;

Dwek, RA ;

Katinger, H ;

Burton, DR ;

Wilson, IA .

SCIENCE, 2003, 300 (5628) :2065-2071

[5] Glycoprotein structural genomics: Solving the glycosylation problem [J].

Chang, Veronica T. ;

Crispin, Max ;

Aricescu, A. Radu ;

Harvey, David J. ;

Nettleship, Joanne E. ;

Fennelly, Janet A. ;

Yu, Chao ;

Boles, Keni S. ;

Evans, Edward J. ;

Stuart, David I. ;

Dwek, Raymond A. ;

Jones, E. Yvonne ;

Owens, Raymond J. ;

Davis, Simon J. .

STRUCTURE, 2007, 15 (03) :267-273

[6] Five Lec1 CHO cell mutants have distinct Mgat1 gene mutations that encode truncated N-acetylglucosaminyltransferase [J].

Chen, W ;

Stanley, P .

GLYCOBIOLOGY, 2003, 13 (01) :43-50

[7] Genetic remodeling of protein glycosylation in vivo induces autoimmune disease [J].

Chui, D ;

Sellakumar, G ;

Green, RS ;

Sutton-Smith, M ;

McQuistan, T ;

Marek, KW ;

Morris, HR ;

Dell, A ;

Marth, JD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (03) :1142-1147

[8] Inhibition of hybrid-and complex-type glycosylation reveals the presence of the GlcNAc transferase I-independent fucosylation pathway [J].

Crispin, Max ;

Harvey, David J. ;

Chang, Veronica T. ;

Yu, Chao ;

Aricescu, A. Radu ;

Jones, E. Yvonne ;

Davis, Simon J. ;

Dwek, Raymond A. ;

Rudd, Pauline M. .

GLYCOBIOLOGY, 2006, 16 (08) :748-756

[9] Monoglucosylated glycans in the secreted human complement component C3: implications for protein blosynthesis and structure [J].

Crispin, MDM ;

Ritchie, GE ;

Critchley, AJ ;

Morgan, BP ;

Wilson, IA ;

Dwek, RA ;

Sim, RB ;

Rudd, PM .

FEBS LETTERS, 2004, 566 (1-3) :270-274

[10] A SYSTEMATIC NOMENCLATURE FOR CARBOHYDRATE FRAGMENTATIONS IN FAB-MS MS SPECTRA OF GLYCOCONJUGATES [J].

DOMON, B ;

COSTELLO, CE .

GLYCOCONJUGATE JOURNAL, 1988, 5 (04) :397-409

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