In vitro cytokine production by mononuclear cells exposed to bone cement extracts

被引:29
作者
Granchi, D
Ciapetti, G
Filippini, F
Stea, S
Cenni, E
Pizzoferrato, A
Toni, A
机构
[1] Ist Ortoped Rizzoli, Lab Fisiopatol Impianti Ortopedici, I-40136 Bologna, Italy
[2] Ist Ortoped Rizzoli, Lab Tecnol Med, I-40136 Bologna, Italy
关键词
acrylic cements; bone resorption; aseptic loosening of hip prosthesis; cytokines;
D O I
10.1016/S0142-9612(00)00070-3
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The authors evaluated the ability of bone cement to modify the profile of pro-inflammatory cytokines secreted by the immune cells. Peripheral blood mononuclear cells (PBMC) collected from healthy individuals were cultured with cement extracts and tested to assess the release of IL-1 beta, TNF alpha, GM-CSF and IL-6 in both unstimulated and PHA-stimulated PBMC. The cytokine release of unstimulated PBMC was very poor, and in particular the IL-1 beta was undetectable: the addition of cement extract increased both TNF alpha and GM-CSF release and decreased IL-6, sometimes significantly. The most recurrent observation in PHA-stimulated PBMCs exposed to bone cement extract was the increase in both IL-1 beta and IL-6 release, while both the mean concentration and the index of release of TNF alpha and GM-CSF were changeable. In conclusion our results showed that leachable components of some bone cements can induce in vitro the release of pro-inflammatory cytokines which are known to be involved in the bone resorption associated with aseptic loosening of hip prostheses. These findings allowed us to identify materials endowed with the highest inflammatory power. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:1789 / 1795
页数:7
相关论文
共 36 条
  • [1] AKATSU T, 1991, J BONE MINER RES, V6, P183
  • [2] DIFFERENTIAL ACTIVITY OF GRANULOCYTE-MACROPHAGE AND MACROPHAGE COLONY STIMULATING FACTORS ON BONE-RESORPTION IN FETAL-RAT LONG-BONE ORGAN-CULTURES
    BERTOLINI, DR
    STRASSMANN, G
    [J]. CYTOKINE, 1991, 3 (05) : 421 - 427
  • [3] BOYUM A, 1968, SCAND J CLIN LAB INV, VS 21, P9
  • [4] ANCHORAGE OF THE FEMORAL HEAD PROSTHESIS TO THE SHAFT OF THE FEMUR
    CHARNLEY, J
    [J]. JOURNAL OF BONE AND JOINT SURGERY-BRITISH VOLUME, 1960, 42 (01): : 28 - 30
  • [5] CIAPETTI G, 2000, IN PRESS J BIOMAT SC
  • [6] *EN ISO, 1996, 10993 EN ISO
  • [7] FDA, 1999, INITI PHARM, P1
  • [8] LYMPHOCYTE-RESPONSE TO POLYMETHYLMETHACRYLATE IN LOOSE TOTAL HIP PROSTHESES
    GILALBAROVA, J
    LACLERIGA, A
    BARRIOS, C
    CANADELL, J
    [J]. JOURNAL OF BONE AND JOINT SURGERY-BRITISH VOLUME, 1992, 74 (06): : 825 - 830
  • [9] Loosening and osteolysis of cemented joint arthroplasties - A biologic spectrum
    Goodman, SB
    Huie, P
    Song, Y
    Lee, K
    Doshi, A
    Rushdieh, B
    Woolson, S
    Maloney, W
    Schurman, D
    Sibley, R
    [J]. CLINICAL ORTHOPAEDICS AND RELATED RESEARCH, 1997, (337) : 149 - 163
  • [10] Cellular profile and cytokine production at prosthetic interfaces - Study of tissues retrieved from revised hip and knee replacements
    Goodman, SB
    Huie, P
    Song, Y
    Schurman, D
    Maloney, W
    Woolson, S
    Sibley, R
    [J]. JOURNAL OF BONE AND JOINT SURGERY-BRITISH VOLUME, 1998, 80B (03): : 531 - 539