Simple application of adipose-derived stem cell-derived extracellular vesicles coating enhances cytocompatibility and osteoinductivity of titanium implant

被引：22

作者：

Chen, Lifeng ^{[1
,2
]}

Mou, Shan ^{[1
,2
]}

Hou, Jinfei ^{[1
,2
]}

Fang, Huimin ^{[1
,2
]}

Zeng, Yuyang ^{[1
,2
]}

Sun, Jiaming ^{[1
,2
]}

Wang, Zhenxing ^{[1
,2
]}

机构：

[1] Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Dept Plast Surg, Wuhan 430022, Peoples R China

[2] Huazhong Univ Sci & Technol, Wuhan Union Hosp, Wuhan Clin Res Ctr Superficial Organ Reconstruct, 1277 Jiefang Ave, Wuhan 430022, Peoples R China

来源：

REGENERATIVE BIOMATERIALS | 2021年 / 8卷 / 01期

基金：

国家重点研发计划; 中国国家自然科学基金;

关键词：

adipose-derived stem cell-derived extracellular vesicles; titanium surface modification; fibronectin; osteoinductivity; IN-VITRO; DIFFERENTIATION; VIVO; OSSEOINTEGRATION; TISSUE;

D O I：

10.1093/rb/rbaa038

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

082905 [生物质能源与材料]; 100103 [病原生物学];

摘要：

Surface modification using bioactive molecules is frequently performed to improve the biological properties of medical metal biomaterial titanium (Ti) implants. Developmental evidence suggests that mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) served as potent bioactive component. As a subset of MSC-EV, adipose-derived stem cell-derived extracellular vesicles (ADSC-EVs) could be obtained from abundant adipose tissue. Meanwhile, it possesses multiple regenerative properties and might be used to endow biological activities to medical Ti implant. Here, we present a simple ADSC-EV coating strategy based on physisorption of fibronectin. This ADSC-EV functionalized Ti implants (EV-Ti) revealed enhanced osteoblast compatibility and osteoinductive activity. Cell spreading area of EV-Ti group was 1.62- and 1.48-fold larger than that of Ti group after 6 and 12 h of cell seeding, respectively. Moreover, EV-Ti promoted alkaline phosphatase, collagen 1 and osteocalcin gene expression in osteoblast by 1.51-, 1.68- and 1.82-fold compared with pristine Ti, respectively. Thus, the MSC-EVs modification method reported here provide a clinically translatable strategy to promote the bioactivity of Ti implants.

引用

页数：9

共 38 条

[1]

ADELL R, 1970, Scandinavian Journal of Plastic and Reconstructive Surgery, V4, P19

[2]

OSSEOINTEGRATED TITANIUM IMPLANTS - REQUIREMENTS FOR ENSURING A LONG-LASTING, DIRECT BONE-TO-IMPLANT ANCHORAGE IN MAN [J].