Multicolour hybrid nanoprobes of molecular beacon conjugated quantum dots: FRET and gel electrophoresis assisted target DNA detection

被引：45

作者：

Kim, Joong Hyun

Chaudhary, Sumit

Ozkan, Mihrimah ^{[1
]}

机构：

[1] Univ Calif Riverside, Dept Elect Engn, Riverside, CA 92521 USA

[2] Univ Calif Santa Barbara, California Nanosyst Informat, Santa Barbara, CA 93106 USA

[3] Univ Calif Riverside, Ctr Nanoscale Sci & Engn, Riverside, CA 92521 USA

来源：

NANOTECHNOLOGY | 2007年 / 18卷 / 19期

关键词：

D O I：

10.1088/0957-4484/18/19/195105

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

We have developed multicolour hybrid DNA probes employing green, yellow and orange colour quantum dot conjugated molecular beacons with black hole quencher 2. Optical and electrophoretic characterization revealed fluorescent energy transfer that follows the FRET mechanism with single nucleotide discrimination. Target DNA identification was observed to be highly sensitive up to 8 ng in gel electrophoresis. Comparison with the conventional organic dye SYBR Gold (TM) showed that our hybrid nanoprobes exhibit more stable performance with less background signal.

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页数：7

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