Isolation and expression of a human SRY-related cDNA, hSOX20

被引：14

作者：

Hiraoka, Y

Ogawa, M

Sakai, Y

Taniguchi, K

Fujii, T

Umezawa, A

Hata, J

Aiso, S

机构：

[1] Keio Univ, Sch Med, Dept Anat, Shinjuku Ku, Tokyo 160, Japan

[2] Keio Univ, Sch Med, Dept Obstet & Gynecol, Shinjuku Ku, Tokyo 160, Japan

[3] Keio Univ, Sch Med, Dept Pathol, Shinjuku Ku, Tokyo 160, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1998年 / 1396卷 / 02期

关键词：

DNA binding; embryonic carcinoma; fetal testis; HMG box; SOX;

D O I：

10.1016/S0167-4781(97)00186-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

SOX is a family of genes related to the testis-determining gene, SRY. We have isolated and sequenced an hSOX20 cDNA from a cell line of human embryonic carcinoma, This cDNA contains an open reading frame (ORF) encoding 233 amino acids. The protein encompasses an SRY-type HMG box exhibiting strong homologies to those of mouse Sox15 and Sox16. Various adult and fetal tissues were tested for hSOX20 mRNA by Northern analysis. Its expression is restricted to the fetal testis and the size of the transcript is 1.5 knt, Electrophoretic mobility shift assay indicated that recombinant hSOX20 polypeptide is capable of binding to AACAAT sequence. (C) 1998 Elsevier Science B.V.

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页码：132 / 137

页数：6

相关论文

共 38 条

[1] THE SRY-RELATED HMG BOX-CONTAINING GENE SOX6 IS EXPRESSED IN THE ADULT TESTIS AND DEVELOPING NERVOUS-SYSTEM OF THE MOUSE [J].

CONNOR, F ;

WRIGHT, E ;

DENNY, P ;

KOOPMAN, P ;

ASHWORTH, A .

NUCLEIC ACIDS RESEARCH, 1995, 23 (17) :3365-3372

[2] AN SRY-RELATED GENE EXPRESSED DURING SPERMATOGENESIS IN THE MOUSE ENCODES A SEQUENCE-SPECIFIC DNA-BINDING PROTEIN [J].

DENNY, P ;

SWIFT, S ;

CONNOR, F ;

ASHWORTH, A .

EMBO JOURNAL, 1992, 11 (10) :3705-3712

[3] A CONSERVED FAMILY OF GENES RELATED TO THE TESTIS DETERMINING GENE, SRY [J].

DENNY, P ;

SWIFT, S ;

BRAND, N ;

DABHADE, N ;

BARTON, P ;

ASHWORTH, A .

NUCLEIC ACIDS RESEARCH, 1992, 20 (11) :2887-2887

[4] SEQUENCE AND EXPRESSION OF SOX-18 ENCODING A NEW HMG-BOX TRANSCRIPTION FACTOR [J].

DUNN, TL ;

MYNETTJOHNSON, L ;

WRIGHT, EM ;

HOSKING, BM ;

KOOPMAN, PA ;

MUSCAT, GEO .

GENE, 1995, 161 (02) :223-225

[5] SRY, LIKE HMG1, RECOGNIZES SHARP ANGLES IN DNA [J].

FERRARI, S ;

HARLEY, VR ;

PONTIGGIA, A ;

GOODFELLOW, PN ;

LOVELLBADGE, R ;

BIANCHI, ME .

EMBO JOURNAL, 1992, 11 (12) :4497-4506

[6] CAMPOMELIC DYSPLASIA AND AUTOSOMAL SEX REVERSAL CAUSED BY MUTATIONS IN AN SRY-RELATED GENE [J].

FOSTER, JW ;

DOMINGUEZSTEGLICH, MA ;

GUIOLI, S ;

KWOK, C ;

WELLER, PA ;

STEVANOVIC, M ;

WEISSENBACH, J ;

MANSOUR, S ;

YOUNG, ID ;

GOODFELLOW, PN ;

BROOK, JD ;

SCHAFER, AJ .

NATURE, 1994, 372 (6506) :525-530

[7] THE HMG DOMAIN OF LYMPHOID ENHANCER FACTOR-I BENDS DNA AND FACILITATES ASSEMBLY OF FUNCTIONAL NUCLEOPROTEIN STRUCTURES [J].

GIESE, K ;

COX, J ;

GROSSCHEDL, R .

CELL, 1992, 69 (01) :185-195

[8] PARTIAL CLONING OF SOX-11 AND SOX-12, 2 NEW HUMAN SOX GENES [J].

GOZE, C ;

POULAT, F ;

BERTA, P .

NUCLEIC ACIDS RESEARCH, 1993, 21 (12) :2943-2943

[9] A GENE-MAPPING TO THE SEX-DETERMINING REGION OF THE MOUSE Y-CHROMOSOME IS A MEMBER OF A NOVEL FAMILY OF EMBRYONICALLY EXPRESSED GENES [J].

GUBBAY, J ;

COLLIGNON, J ;

KOOPMAN, P ;

CAPEL, B ;

ECONOMOU, A ;

MUNSTERBERG, A ;

VIVIAN, N ;

GOODFELLOW, P ;

LOVELLBADGE, R .

NATURE, 1990, 346 (6281) :245-250

[10] MOLECULAR-BASIS OF MAMMALIAN SEXUAL DETERMINATION - ACTIVATION OF MULLERIAN-INHIBITING SUBSTANCE GENE-EXPRESSION BY SRY [J].

HAQQ, CM ;

KING, CY ;

UKIYAMA, E ;

FALSAFI, S ;

HAQQ, TN ;

DONAHOE, PK ;

WEISS, MA .

SCIENCE, 1994, 266 (5190) :1494-1500

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