Megavesicles implicated in the rapid transport of intracisternal aggregates across the Golgi stack

被引：83

作者：

Volchuk, A

Amherdt, M

Ravazzola, M

Brügger, B

Rivera, VM

Clackson, T

Perrelet, A

Söllner, TH

Rothman, JE

Orci, L

机构：

[1] Mem Sloan Kettering Canc Ctr, Cellular Biochem & Biophys Program, New York, NY 10021 USA

[2] Univ Geneva, Sch Med, Dept Morphol, CH-1211 Geneva 4, Switzerland

[3] ARIAD Pharmaceut Inc, Cambridge, MA 02139 USA

来源：

CELL | 2000年 / 102卷 / 03期

基金：

英国医学研究理事会; 美国国家卫生研究院;

关键词：

D O I：

10.1016/S0092-8674(00)00039-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Engineered protein aggregates ranging up to 400 nm in diameter were selectively deposited within the cis-most cisternae of the Golgi stack following a 15 degrees C block. These aggregates are much larger than the standard volume of Golgi vesicles, yet they are transported across the stack within 10 min after warming the cells to 20 degrees C. Serial sectioning reveals that during the peak of anterograde transport, about 20% of the aggregates were enclosed in topologically free "megavesicles" which appear to pinch off from the rims of the cisternae. These megavesicles can explain the rapid transport of aggregates without cisternal progression on this time scale.

引用

收藏

页码：335 / 348

页数：14

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