Separation and quantitation of phospholipids and lysophospholipids by high-performance liquid chromatography

被引：80

作者：

Lesnefsky, EJ ^{[1
]}

Stoll, MSK

Minkler, PE

Hoppel, CL

机构：

[1] Case Western Reserve Univ, Dept Med, Div Cardiol, Cleveland, OH 44106 USA

[2] Case Western Reserve Univ, Dept Med, Div Clin Pharmacol, Cleveland, OH 44106 USA

[3] Case Western Reserve Univ, Dept Pharmacol, Cleveland, OH 44106 USA

[4] Louis Stokes Vet Affairs Med Ctr, Ctr Geriatr Res Educ & Clin, Cleveland, OH 44106 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2000年 / 285卷 / 02期

关键词：

high-performance liquid chromatography; cardiolipin; mass spectrometry;

D O I：

10.1006/abio.2000.4783

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a comprehensive approach to the separation, quantitation, and characterization of phospholipids and lysophospholipids present in complex biological samples. The central feature is a normal-phase HPLC separation of individual phospholipid and lysophospholipid classes. In this single chromatographic step, phospholipids and lysophospholipids are separated and recovered for quantitation by organic phosphate assay and characterization by acyl-group composition. Recovery of phospholipids and lysophospholipids from HPLC averages 80-90%. Isolated phospholipid and lysophospholipid fractions are available for separation of individual molecular species by second-dimension reverse-phase HPLC and characterization of individual molecular species by mass spectrometry. (C) 2000 Academic Press.

引用

页码：246 / 254

页数：9

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