Purification, chemical modification and immunostimulating activity of polysaccharides from Tremella aurantialba fruit bodies

被引：29

作者：

Du, Xiu-ju ^{[1
,2
]}

Zhang, Jing-song ^{[1
]}

Yang, Yan ^{[1
]}

Tang, Qing-jiu ^{[1
]}

Jia, Wei ^{[1
]}

Pan, Ying-jie ^{[3
]}

机构：

[1] Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai 201106, Peoples R China

[2] Liaocheng Univ, Coll Life Sci, Liaocheng 252059, Peoples R China

[3] Shanghai Ocean Univ, Coll Food Sci, Shanghai 200090, Peoples R China

来源：

JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B | 2010年 / 11卷 / 06期

关键词：

Tremella aurantialba; Purification; Polysaccharide; Sulfation; Immunostimulating activity; ANTICOAGULANT;

D O I：

10.1631/jzus.B0900402

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ultrafiltration and a series of chromatographic steps were used to isolate and purify polysaccharides from Tremella aurantialba fruit bodies. Three crude fractions (TAP50w, TAP10-50w, and TAP1-10w), five semi-purified fractions (TAPA-TAPE), and one purified fraction (TAPA1) were obtained. A sulfated derivative of TAPA1 (TAPA1-s) was prepared by chemical modification. The immunostimulating activity of the polysaccharide fractions in vitro was determined using the mouse spleen lymphocyte proliferation assay. Of the three crude fractions tested, cell proliferation rates were increased most by TAP50w. Furthermore, TAPA1-s was markedly more stimulatory than TAPA1, indicating that sulfonation was an effective way to enhance the immunostimulating activity of polysaccharide.

引用

页码：437 / 442

页数：6

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