The fluorescence resonance energy transfer (FRET) gate: A time-resolved study

被引：87

作者：

Xu, QH

Wang, S

Korystov, D

Mikhailovsky, A

Bazan, GC

Moses, D

Heeger, AJ ^{[1
]}

机构：

[1] Univ Calif Santa Barbara, Inst Polymers & Organ Solids, Santa Barbara, CA 93106 USA

[2] Univ Calif Santa Barbara, Dept Chem, Santa Barbara, CA 93106 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2005年 / 102卷 / 03期

关键词：

conjugated polymers; DNA sensors; time-resolved anisotropy; ultrafast spectroscopy;

D O I：

10.1073/pnas.0408568102

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The two-step energy-transfer process in a self-assembled complex comprising a cationic conjugated polymer (CCP) and a dsDNA is investigated by using pump-clump-emission spectroscopy and time-correlated single-photon counting; energy is transferred from the CCP to an ethiclium bromide (EB) molecule intercalated into the dsDNA through a fluorescein molecule linked to one terminus of the DNA. Time-dependent anisotropy measurements indicate that the inefficient direct energy transfer from the CCP to the intercalated EB results from the near orthogonality of their transition moments. These measurements also show that the transition moment of the fluorescein spans a range of angular distributions and lies between that of the CCP and EB. Consequently, the fluorescein acts as a fluorescence resonance energy-transfer gate to relay the excitation energy from the CCP to the EB.

引用

页码：530 / 535

页数：6

共 20 条

[1] INTERACTION OF ETHIDIUM BROMIDE WITH WHOLE AND SELECTIVELY DEPROTEINIZED DEOXYNUCLEOPROTEINS FROM CALF THYMUS [J].