Aspergillus fumigatus alb1 encodes naphthopyrone synthase when expressed in Aspergillus oryzae

被引：24

作者：

Watanabe, A

Fujii, I

Tsai, HF

Chang, YC

Kwon-Chung, KJ

Ebizuka, Y ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Tokyo 1130033, Japan

[2] NIAID, Clin Invest Lab, Bethesda, MD 20892 USA

来源：

FEMS MICROBIOLOGY LETTERS | 2000年 / 192卷 / 01期

基金：

日本学术振兴会;

关键词：

melanin; biosynthesis; polyketide synthase; naphthopyrone; tetrahydroxynaphthalene;

D O I：

10.1016/S0378-1097(00)00402-X

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学]; 100705 [微生物与生化药学];

摘要：

Conidial pigment biosynthesis is an important virulence factor in Aspergillus fumigatus, a human fungal pathogen. Involvement of DHN-melanin pathway in the biosynthesis of A. fumigatus conidial pigment implies that the Alb1p polyketide synthase (PKS) is a 1,3,6,8-tetrahydroxynaphthalene (T4HN) synthase. The Alb1p, however, shows higher sequence similarity to a naphthopyrone synthase than to a T4HN synthase. To clarify the function of Alb1p, the alb1 gene was overexpressed in a heterologous host Aspergillus oryzae. The Alb1p PKS product in this heterologous expression system was identified as heptaketide naphthopyrone instead of pentaketide T4HN. The data suggest that Alb1p is a naphthopyrone synthase. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：39 / 44

页数：6

共 28 条

[1]

SEQUENCE AND MOLECULAR-STRUCTURE OF THE ASPERGILLUS-NIDULANS YA (LACCASE-I) GENE [J].