Activity-based protein profiling for type I methionine aminopeptidase by using photo-affinity trimodular probes

被引：22

作者：

Qiu, Wen-Wei ^{[1
]}

Xu, Jie ^{[1
]}

Li, Jing-Ya ^{[1
]}

Li, Jia ^{[1
]}

Nana, Fa-Jun ^{[1
]}

机构：

[1] Shanghai Inst Mat Med, Chinese Natl Ctr Drug Screening, Shanghai 201203, Peoples R China

来源：

CHEMBIOCHEM | 2007年 / 8卷 / 12期

关键词：

azido-acetyl group; molecular recognition; photoaffinity labeling; protein profiling; trimodular probes;

D O I：

10.1002/cbic.200700148

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(Chemical Equation Presented) Three in one. A trimodular probe (1) with three functional groups (for target recognition, proximal target crosslinking, and distal reporter tag attachment via click chemistry of a photostable azido-acetyl group) has been designed. We demonstrate its specificity, sensitivity, and potential for general application in activity-based protein profiling for type I methionine aminopeptidases. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：1351 / 1358

页数：8

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