Pervasive combinatorial modification of histone H3 in human cells

被引：179

作者：

Garcia, Benjamin A.

Pesavento, James J.

Mizzen, Craig A. ^{[1
]}

Kelleher, Neil L.

机构：

[1] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA

[2] Univ Illinois, Ctr Biophys & Comp Biol, Urbana, IL 61801 USA

[3] Univ Illinois, Dept Cell & Dev Biol, Urbana, IL 61801 USA

[4] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

来源：

NATURE METHODS | 2007年 / 4卷 / 06期

关键词：

D O I：

10.1038/nmeth1052

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed a platform using hydrophilic interaction chromatography and high-resolution tandem mass spectrometry (MS) for analyses of histone H3 that allows comprehensive characterization of 'histone codes' at the molecular level. We identified over 150 differentially modified forms of histone H3.2 in asynchronously grown and butyrate-treated HeLa cells, revealing pervasive combinatorial modification previously unaccounted for by other techniques and providing a clarified estimate of the molecular diversity of histone H3 in mammals.

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页码：487 / 489

页数：3

相关论文

共 15 条

[1] Genomic maps and comparative analysis of histone modifications in human and mouse [J].

Bernstein, BE ;

Kamal, M ;

Lindblad-Toh, K ;

Bekiranov, S ;

Bailey, DK ;

Huebert, DJ ;

McMahon, S ;

Karlsson, EK ;

Kulbokas, EJ ;

Gingeras, TR ;

Schreiber, SL ;

Lander, ES .

CELL, 2005, 120 (02) :169-181

[2] Protein identification using sequential ion/ion reactions and tandem mass spectrometry [J].

Coon, JJ ;

Ueberheide, B ;

Syka, JEP ;

Dryhurst, DD ;

Ausio, J ;

Shabanowitz, J ;

Hunt, DF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (27) :9463-9468

[3] Translating the histone code [J].

Jenuwein, T ;

Allis, CD .

SCIENCE, 2001, 293 (5532) :1074-1080

[4] Separation of acetylated core histones by hydrophilic-interaction liquid chromatography [J].

Lindner, H ;

Sarg, B ;

Meraner, C ;

Helliger, W .

JOURNAL OF CHROMATOGRAPHY A, 1996, 743 (01) :137-144

[5] PTMs on H3 variants before chromatin assembly potentiate their final epigenetic state [J].

Loyola, Alejandra ;

Bonaldi, Tiziana ;

Roche, Daniele ;

Imhof, Axel ;

Almouzni, Genevieve .

MOLECULAR CELL, 2006, 24 (02) :309-316

[6] The key to development: interpreting the histone code? [J].

Margueron, R ;

Trojer, P ;

Reinberg, D .

CURRENT OPINION IN GENETICS & DEVELOPMENT, 2005, 15 (02) :163-176

[7] Resolution of allelic and non-allelic variants of histone H1 by cation-exchange-hydrophilic-interaction chromatography [J].

Mizzen, CA ;

Alpert, AJ ;

Levesque, L ;

Kruck, TPA ;

McLachlan, DR .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2000, 744 (01) :33-46

[8] Quantitative analysis of modified proteins and their positional isomers by tandem mass spectrometry: Human histone H4 [J].

Pesavento, James J. ;

Mizzen, Craig A. ;

Kelleher, Neil L. .

ANALYTICAL CHEMISTRY, 2006, 78 (13) :4271-4280

[9] Shotgun annotation of histone modifications: A new approach for streamlined characterization of proteins by top down mass spectrometry [J].

Pesavento, JJ ;

Kim, YB ;

Taylor, GK ;

Kelleher, NL .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (11) :3386-3387

[10] ING2 PHD domain links histone H3 lysine 4 methylation to active gene repression [J].

Shi, Xiaobing ;

Hong, Tao ;

Walter, Kay L. ;

Ewalt, Mark ;

Michishita, Eriko ;

Hung, Tiffany ;

Carney, Dylan ;

Pena, Pedro ;

Lan, Fei ;

Kaadige, Mohan R. ;

Lacoste, Nicolas ;

Cayrou, Christelle ;

Davrazou, Foteini ;

Saha, Anjanabha ;

Cairns, Bradley R. ;

Ayer, Donald E. ;

Kutateladze, Tatiana G. ;

Shi, Yang ;

Cote, Jacques ;

Chua, Katrin F. ;

Gozani, Or .

NATURE, 2006, 442 (7098) :96-99